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Inducing substances for chondrogenic differentiation of dental pulp stem cells in the conditioned medium of a novel chordoma cell line

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Abstract

We successfully established a chordoma cell line, designated TSK-CHO1, derived from the clival chordoma. Currently, there is only one skull base chordoma cell line, UM-chor1, freely available to researchers. The established TSK-CHO1 cells were neoplastic, exhibited pleomorphic features, and secreted brachyury, as revealed by immunocytochemical staining or ELISA of conditioned medium (CM). Cells also secreted SOX9, which enhanced brachyury production. The CM of TSK-CHO1 cells promoted the production of hyaluronic acid and type II collagen during differentiation of human dental pulp stem cells (DPSCs) into fibrocartilage cells. Culture of DPSC pellets in a growth medium supplemented with 10% CM of TSK-CHO1 cells for 2 weeks resulted in the induction of fibrocartilage tissue under normoxic conditions. Brachyury produced by TSK-CHO1 cells promoted the production of collagen type II, peculiar to cartilage, in a dose-dependent manner. The newly established skull base chordoma cell line, TSK-CHO1, is expected to be used for elucidating the pathogenesis of skull base chordoma and for investigating the mechanism underlying the production of fibrocartilage.

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Acknowledgements

This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (no. 18K08987 to S.T. and no. 21K16625 to H.K.), the Japan Brain Foundation (to S.T.), and the Japanese Foundation for Multidisciplinary Treatment of Cancer (to S.T.).

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Authors and Affiliations

  1. Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan

    Hiroyoshi Kino, Hiroyoshi Akutsu, Shingo Takano, Takuma Hara, Eiichi Ishikawa, Yuji Matsumaru & Akira Matsumura

  2. Department of Neurosurgery, Dokkyo Medical University, Mibu, Tochigi, Japan

    Hiroyoshi Akutsu

  3. Laboratory of Clinical Regenerative Medicine, Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan

    Hiroshi Ishikawa & Junko Toyomura

  4. Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan

    Shohei Takaoka & Hiroki Bukawa

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  1. Hiroyoshi Kino
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Correspondence to Hiroyoshi Akutsu.

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The study was approved by the Clinical Ethics Committee of the University of Tsukuba Hospital. Approval number is R01-232, H29-173, R01-178.

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Kino, H., Akutsu, H., Ishikawa, H. et al. Inducing substances for chondrogenic differentiation of dental pulp stem cells in the conditioned medium of a novel chordoma cell line. Human Cell 35, 745–755 (2022). https://doi.org/10.1007/s13577-021-00662-5

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