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Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies

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Abstract

Present study was undertaken to compare various routine laboratory diagnostic tests for rabies detection. Seller’s staining, mouse inoculation test (MIT), Dot-ELISA, Agar gel precipitation test (AGPT) and counter immunoelectrophoresis test (CIET) were the main basic tests performed in the laboratory for the rabies diagnosis. Out of 200 brain specimens, Negri bodies were observed in 52 brain samples by Seller’s staining. Rabies virus was isolated in 56 samples by intra-cerebral inoculation in newborn Swiss-albino mice. Dot-ELISA and AGPT could detect rabies antigen in 55 and 57 samples respectively. Comparative analysis revealed that the CIET is the most sensitive and rapid test among performed diagnostic tests.

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Authors and Affiliations

  1. Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, MS, India

    S. S. Kadam

  2. Department of Microbiology, Bombay Veterinary College, Parel, Mumbai, 400 012, MS, India

    A. A. Sherikar

  3. Laxman Devram Sonawane College, Bhiwandi-Murbad Road, Wadeghar, Kalyan (W), 421301, MS, India

    V. S. **ale

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  1. S. S. Kadam
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Correspondence to S. S. Kadam.

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Kadam, S.S., Sherikar, A.A. & **ale, V.S. Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies. Indian J. Virol. 22, 142–145 (2011). https://doi.org/10.1007/s13337-011-0052-1

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