SpringerLink
Log in

Isolation and characterization of polymorphic microsatellite loci in the grand jackknife clam Solen grandis (Bivalvia: Veneroida)

  • Research Article
  • Published:
Genes & Genomics Aims and scope Submit manuscript

Abstract

The grand jackknife clam Solen grandis (Dunker, 1861) is an overexploited bivalve species. In order to promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genetics. For this purpose, we isolated the first set of 18 polymorphic microsatellite markers and examined their allelic variation using a total of 30 wild individuals collected from one locality. The number of alleles per locus ranged from 5 to 24, and the observed and expected heterozygosity varied from 0.300 to 0.967 and from 0.721 to 0.952, respectively. The high variabilities at these loci indicated that they would be useful tool for exploring the population genetics of S. grandis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Carlsson J, Morrison CL and Reece KS (2006) Wild and aquaculture populations of the eastern oyster compared using microsatellites. J. Hered. 97: 595–598.

    Article  CAS  PubMed  Google Scholar 

  • Chapuis MP and Estoup A (2007) Microsatellite null alleles and estimation of population differentiation. Mol. Biol. Evol. 24: 621–631.

    Article  CAS  PubMed  Google Scholar 

  • Chapuis MP, Loiseau A, Michalakis Y, Lecoq M and Estoup A (2005) Characterization and PCR multiplexing of polymorphic microsatellite loci for the locust Locusta migratoria. Mol. Ecol. Notes 5: 554–557.

    Article  CAS  Google Scholar 

  • Chen AH, Yao GX and Zhang ZW (2009) Studies on artificial propagation of Solen grandis (Dunker) in pilot scale. Mar. Fish. 31: 66–72.

    Google Scholar 

  • Chistiakov DA, Hellemans B and Volckaert FAM (2006) Microsatellites and their genomic distribution, evolution, function and applications: A review with special reference to fish genetics. Aquaculture 255: 1–29.

    Article  CAS  Google Scholar 

  • Dai CJ (2002) Evaluation on nutrition componets of the soft part in Solen grandis. J. Jimei Univ. 7: 304–308.

    Google Scholar 

  • Dakin EE and Avise JC (2004) Microsatellite null alleles in parentage analysis. Heredity 93: 504–509.

    Article  CAS  PubMed  Google Scholar 

  • De Sousa SN, Finkeldey R and Gailing O (2005) Experimental verification of microsatellite null alleles in Norway spruce (Picea abies [L.] Karst.): implications for population genetic studies. Plant Mol. Biol. Rep. 23: 113–119.

    Article  Google Scholar 

  • Dieringer D and Schlötterer C (2003) Microsatellite analyser (MSA): a platform independent analysis tool for large microsatellite data sets. Mol. Ecol. Notes 3: 167–169.

    Article  CAS  Google Scholar 

  • Gardner MG, Cooper SJB, Bull CM and Grant WN (1999) Isolation of microsatellite loci from a Social Lizard, Egernia stokesii, using a modified enrichment procedure. J. Hered. 90: 301–304.

    Article  CAS  Google Scholar 

  • Jarne P and Lagoda PJL (1996) Microsatellites, from molecules to populations and back. Trend Ecol. Evol. 11: 424–429.

    Article  Google Scholar 

  • Lehmann T, Besanky NJ, Hawley WA, Fahey TG, Kamau L and Collins FH (1997) Microgeographic structure of Anopheles gambiae in western Kenya based on mtDNA and microsatellite loci. Mol. Ecol. 6: 243–253.

    Article  CAS  PubMed  Google Scholar 

  • Li JJ and Li Q (2008) Isolation and characterization of twelve novel microsatellite loci in the ark shell Scapharca broughtonii. Conserv. Genet. 9: 1055–1057.

    Article  CAS  Google Scholar 

  • Li Q, Yu H and Yu RH (2006) Genetic variability assessed by microsatellites in cultured populations of the Pacific oyster (Crassostrea gigas) in China. Aquaculture 259: 95–102.

    Article  CAS  Google Scholar 

  • Liu J, Li Q and Kong LF (2009) Isolation and characterization of 13 microsatellite loci in the pen shell Atrina pectinata (Bivalvia: Pinnidae). Conserv. Genet. 10: 1369–1371.

    Article  CAS  Google Scholar 

  • McGoldrick DJ, Hedgecock D, English LJ, Baoprasertkul P and Ward RD (2000) The transmission of microsatellite alleles in Australian and North American stocks of the Pacific oyster (Crassostrea gigas): selection and null alleles. J. Shellfish Res. 19: 779–788.

    Google Scholar 

  • Naciri Y, Vigouroux Y, Dallas J, Desmarais E, Delsert C and Bonhomme (1995) Identification and inheritance of (GA/TC)n and (AC/GT)n repeats in the European flat oyster Ostrea edulis (L.). Mol. Mar. Biol. Biotechnol. 4: 83–89.

    CAS  PubMed  Google Scholar 

  • Naish KA and Boulding EG (2001) Trinucleotide microsatellite loci for the zebra mussel Dreissena polymorpha, an invasive species in Europe and North America. Mol. Ecol. Notes 1: 286–288.

    Article  CAS  Google Scholar 

  • Pemberton JM, Slate J, Bancroft DR and Barrett JA (1995) Nonamplifying alleles at microsatellite loci: a caution for parentage and population studies. Mol. Ecol. 4: 249–252.

    Article  CAS  PubMed  Google Scholar 

  • Reece KS, Ribeiro WL, Gaffney PM, Carnegie RB and Allen SK Jr (2004) Microsatellite marker development and analysis in the eastern oyster, Crassostrea virginica: confirmation of null alleles and non-Mendelian segregation ratios. J. Hered. 95: 355–361.

    Article  Google Scholar 

  • Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol. Ecol. Resour. 8: 103–106.

    Article  Google Scholar 

  • Rousset F and Raymond M (1995) Testing heterozygote excess and deficiency. Genetics 140: 1413–1419.

    CAS  PubMed  Google Scholar 

  • Rubinsztein DC., Amos W, Leggo J, Goodburn S, Jain S, Li SH, Margolis RL, Ross CA and Ferguson-Smith MA (1995). Microsatellite evolution-evidence for directionality and variation in rate between species. Nature Genet. 10: 337–343.

    Article  CAS  PubMed  Google Scholar 

  • Schlötterer C (2000) Evolutionary dynamics of microsatellite DNA. Chromosoma 109: 365–371.

    Article  PubMed  Google Scholar 

  • Smith C, Benzie JAH and Wilson KJ (2003) Isolation and characterization of eight microsatellite loci from silver-lipped pearl oyster Pinctada maxima. Mol. Ecol. Notes 3: 125–127.

    Article  CAS  Google Scholar 

  • Vadopalas B and Bentzen P (2000) Isolation and characterization of di- and tetranucleotide microsatellite loci in geoduck clams, Panopea abrupta. Mol. Ecol. 9: 1435–1436.

    Article  CAS  PubMed  Google Scholar 

  • Van Oosterhout C, Hutchinson WF, Wills DPM and Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genoty** errors in microsatellite data. Mol. Ecol. Notes 4: 535–538

    Article  Google Scholar 

  • Wilson AB, Boulding EG and Naish KA (1999) Characterization of tri- and tetranucleotide microsatellite loci in the invasive mollusc Dreissena bugensis. Mol. Ecol. 8: 692–693.

    Article  CAS  PubMed  Google Scholar 

  • Yang DJ and Wang YL (1996) Marine Invertebrate in Northern China. High Education Press, Bei**g.

    Google Scholar 

  • Zane L, Bargelloni L and Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol. Ecol. 11: 1–16.

    Article  CAS  PubMed  Google Scholar 

  • Zhang ZW, Yao GX and Chen AH (2009) The tissue specificity and the genetic diversity of the allozymes of Solen grandis Dunker. Mar. Sci. 33: 41–43.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Fisheries College, Ocean University of China, Qingdao, 266003, China

    Yang Yuan, Qi Li, Lingfeng Kong & **aodong Zheng

Authors
  1. Yang Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qi Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lingfeng Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. **aodong Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qi Li.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yuan, Y., Li, Q., Kong, L. et al. Isolation and characterization of polymorphic microsatellite loci in the grand jackknife clam Solen grandis (Bivalvia: Veneroida). Genes Genom 32, 191–197 (2010). https://doi.org/10.1007/s13258-009-0780-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13258-009-0780-4

Keywords

Search

Navigation