SpringerLink
Log in

In vivo bone regeneration evaluation of duck’s feet collagen/PLGA scaffolds in rat calvarial defect

  • Article
  • Published:
Macromolecular Research Aims and scope Submit manuscript

Abstract

Tissue engineered bone substitutes should mimic natural bone characteristics to be highly-suitable for treating bone defects in addition to its biocompatibility and good mechanical stability. In this study, we performed a detailed in vivo bone regeneration evaluation of 80 wt% duck’s feet collagen/poly(lactide-co-glycolide) scaffolds (DC/PLGA) fabricated by solvent casting/salt leaching strategy in a rat calvarial defect as model. We have already shown a strong influence of DC/ PLGA scaffolds on bone regeneration in terms of biomaterial cohesion, architecture, mechanical features, and in vitro biological properties. The as-fabricated scaffold has shown significant increase in osteogenesis, initial bone formation and differentiation, ascribed to the high percentage of DC in the 80 wt% DC/PLGA scaffold. The in vivo implanted scaffold was found be well-attached to the bone defect region and eventually gets integrated with the surrounding tissues without any pronounced inflammatory reactions. Compared to bare PLGA, an increased recovery in bone volume was observed at 8th week post-surgery. Thus, the 80 wt% DC/PLGA scaffold can be envisioned as a potential alternative bone graft in bone tissue engineering.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M. Pei, J. T. Li, D. B. McConda, S. J. Wen, N. B. Clovis, and S. S. Danley, Bone, 78, 1 (2015).

    Article  CAS  Google Scholar 

  2. S. Bhumiratana, W. L. Grayson, A. Castaneda, D. N. Rockwood, E. S. Gil, D. L. Kaplan, and G. Vunjak-Novakovic, Biomaterials, 32, 2812 (2011).

    Article  CAS  Google Scholar 

  3. S. Kuttappan, D. Mathew, and M. B. Nair, Int. J. Biol. Macromol., 93, 1390 (2016).

    Article  CAS  Google Scholar 

  4. L. Nie, D. Chen, J. Fu, S. H. Yang, R. X. Hou, and J. P. Suo, Biochem. Eng. J., 98, 29 (2015).

    Article  CAS  Google Scholar 

  5. C. Dhand, S. T. Ong, N. Dwivedi, S. M. Diaz, J. R. Venugopal, B. Navaneethan, M. H. Fazil, S. Liu, V. Seitz, E. Wintermantel, R. W. Beuerman, S. Ramakrishna, N. K. Verma, and R. Lakshminarayanan, Biomaterials, 104, 323 (2016).

    Article  CAS  Google Scholar 

  6. J. A. Inzana, D. Olvera, S. M. Fuller, J. P. Kelly, O. A. Graeve, E. M. Schwarz, S. L. Kates, and H. A. Awad, Biomaterials, 35, 4026 (2014).

    Article  CAS  Google Scholar 

  7. J. A. Kim, J. Lim, R. Naren, H. S. Yun, and E. K. Park, Acta Biomater., 44, 155 (2016).

    Article  CAS  Google Scholar 

  8. W. Tang, D. Lin, Y. M. Yu, H. Y. Niu, H. Guo, Y. Yuan, and C. S. Liu, Acta Biomater., 32, 309 (2016).

    Article  CAS  Google Scholar 

  9. X. Ren, V. Tu, D. Bischoff, D. W. Weisgerber, M. S. Lewis, D. T. Yamaguchi, T. A. Miller, B. A. Harley, and J. C. Lee, Biomaterials, 89, 67 (2016).

    Article  CAS  Google Scholar 

  10. H. J. Park, O. J. Lee, M. C. Lee, B. M. Moon, H. W. Ju, J. M. Lee, J. H. Kim, D. W. Kim, and C. H. Park, Int. J. Biol. Macromol., 78, 215 (2015).

    Article  CAS  Google Scholar 

  11. Y. Cai, J. Guo, C. Chen, C. Yao, S. M. Chung, J. Yao, I. S. Lee, and X. Kong, Mater. Sci. Eng. C, Mater. Biol. Appl., 70, 148 (2017).

    Article  CAS  Google Scholar 

  12. S. Saravanan, R. S. Leena, and N. Selvamurugan, Int. J. Biol. Macromol., 93, 1354 (2016).

    Article  CAS  Google Scholar 

  13. G. Caetano, R. Violante, A. B. Sant'Ana, A. B. Murashima, M. Domingos, A. Gibson, P. Bartolo, and M. A. Frade, Mater. Lett., 182, 318 (2016).

    Article  CAS  Google Scholar 

  14. G. Tomoaia and R. D. Pasca, Clujul Med., 88, 15 (2015).

    Article  Google Scholar 

  15. X. Ma, Z. He, F. Han, Z. Zhong, L. Chen, and B. Li, Colloids Surf. B: Biointerfaces, 143, 81 (2016).

    Article  CAS  Google Scholar 

  16. M. A. C. Bolaños, J. Buttigieg, and J. C. B. Triana, Mater. Sci. Eng. C, 72, 519 (2017).

    Article  Google Scholar 

  17. J. Elango, J. Zhang, B. Bao, K. Palaniyandi, S. Wang, W. Wenhui, and J. S. Robinson, Int. J. Biol. Macromol., 91, 51 (2016).

    Article  CAS  Google Scholar 

  18. L. C. Mozdzen, A. Vucetic, and B. A. Harley, J. Mech. Behav. Biomed. Mater., 66, 28 (2017).

    Article  CAS  Google Scholar 

  19. R. Cholas, S. K. Padmanabhan, F. Gervaso, G. Udayan, G. Monaco, A. Sannino, and A. Licciulli, Mater. Sci. Eng. C: Mater., 63, 499 (2016).

    Article  CAS  Google Scholar 

  20. A. Khojasteh, F. Fahimipour, M. B. Eslaminejad, M. Jafarian, S. Jahangir, F Bastami, and M. Tahriri, Mater. Sci. Eng. C, 69, 780 (2016).

    Article  CAS  Google Scholar 

  21. J. E. Song, S. E. Lee, S. R. Cha, N. K. Jang, N. Tripathy, R. L. Reis, and G. Khang, J. Biomater. Sci. Polym. Ed., 27, 1495 (2016).

    Article  CAS  Google Scholar 

  22. S. M. Kim, H. M. Kim, H. Kuk, E. Y. Kim, J. E. Song, D. S. Suh, C. H. Park, and G. Khang, Polym. Korea, 39, 837 (2015).

    Article  CAS  Google Scholar 

  23. G. B. Ramirez-Rodriguez, J. M. Delgado-Lopez, M. Iafisco, M. Montesi, M. Sandri, S. Sprio, and A. Tampieri, J. Struct. Biol., 196, 138 (2016).

    Article  CAS  Google Scholar 

  24. Z. Chen, L. Kang, Q. Y. Meng, H. Liu, Z. Wang, Z. Guo, and F. Z. Cui, Mater. Sci. Eng. C, Mater. Biol. Appl., 45, 94 (2014).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept of BIN Convergence Tech, Polymer Fusion Res Center & Dept of Polymer∙Nano Sci Tech, Chonbuk National Univ, 567 Baekje-daero, Jeonju, Jeonbuk, 54896, Korea

    Jeong Eun Song, Nirmalya Tripathy, Jae Hun Shin, Dae Hoon Lee, Jae Geun Cha & Gilson Khang

  2. Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, School of Medicine, Hallym University, 77, Sakju-ro, Chuncheon, Gangwon, 24253, Korea

    Chan Hum Park

  3. Sewon Cellontech, 801, Wooyoung Techno Center, 273-15 Seongsu, 2ga 3-dong, Seongdong gu, Seoul, 04783, Korea

    Dong Sam Suh

Authors
  1. Jeong Eun Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nirmalya Tripathy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jae Hun Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dae Hoon Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jae Geun Cha
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chan Hum Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Dong Sam Suh
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Gilson Khang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gilson Khang.

Additional information

Acknowledgments: This research was supported by Technology Commercialization Support Program (814005-03-3-HD020), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Song, J.E., Tripathy, N., Shin, J.H. et al. In vivo bone regeneration evaluation of duck’s feet collagen/PLGA scaffolds in rat calvarial defect. Macromol. Res. 25, 994–999 (2017). https://doi.org/10.1007/s13233-017-5134-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13233-017-5134-5

Keywords

Search

Navigation