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The application of deep eutectic solvent on the extraction and in vitro antioxidant activity of rutin from Sophora japonica bud

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Abstract

The extraction conditions and antioxidant activities of rutin from Sophora japonica bud by deep eutectic solvents were investigated. Box–Behnken design was used to optimize the extraction conditions and the scavenging activities of DPPH, O2− and ·OH of purified rutin were evaluated. The highest yield of 279.8 mg/g was achieved in the extraction medium of choline chloride/triethlene glycol (1/4) under the optimum conditions: water content of the DES 18.1%, extraction time 28.3 min, extraction temperature 70 °C and liquid–solid ratio 10 mg/1 g. The highest extraction amount was slightly different from the predicted value of the established second-order polynomial equation. In addition, The EC50 of DPPH scavenging, O2− scavenging and ·OH scavenging of rutin were 5.68 µg/mL, 0.19 and 0.28 mg/mL, respectively. The above results indicate rutin extracted by the choline chloride/triethylene glycol has excellent antioxidant activity and was an admirable free radical scavenger.

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Acknowledgements

We wish to thank the National Natural Science Foundation of China (21676104; 21336002; 21376096), the Open Funding Project of the State Key Laboratory of Bioreactor Engineering, and the Program of State Key Laboratory of Pulp and Paper Engineering (2017ZD05) for partially funding this work.

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Authors and Affiliations

  1. Laboratory of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China

    Fei Peng, Pei Xu, Bing-Yi Zhao & Wen-Yong Lou

  2. Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou, 510640, China

    Fei Peng, Pei Xu, Bing-Yi Zhao, Min-Hua Zong & Wen-Yong Lou

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  1. Fei Peng
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  2. Pei Xu
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  3. Bing-Yi Zhao
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Correspondence to Wen-Yong Lou.

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Peng, F., Xu, P., Zhao, BY. et al. The application of deep eutectic solvent on the extraction and in vitro antioxidant activity of rutin from Sophora japonica bud. J Food Sci Technol 55, 2326–2333 (2018). https://doi.org/10.1007/s13197-018-3151-9

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  • DOI: https://doi.org/10.1007/s13197-018-3151-9

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