Abstract
Eight polymorphic microsatellite loci are described for the endangered Amur ratsnake, Elaphe schrenckii (Colubridae). Loci were screened in 31 unrelated individuals of E. schrenckii collected from Liaoning Province, China. These markers revealed a high degree of genetic diversity (6–20 alleles per locus) and heterozygosity (H O ranged from 0.296 to 1.000, and H E ranged from 0.469 to 0.850). No significant linkage disequilibrium between pairs of loci was found, except one of them significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. These loci are the first time to be characterized and will be useful for population genetic structure investigation, conservation management and paternity identification study for E. schrenckii.
References
An J, Kim MJ, Park D, Lee J, Krukov V, Kim KS, Lee H, Min MS (2010) Development of 10 microsatellite loci from the Korean Ratsnake (Elaphe schrenckii) and its application across Elaphe species from South Korea, Russia, and China. Genes Genomics 32:401–405
Huang MH (1998) Ptyas Fitzinger, 1833. In: Zhao EM, Huang MH, Zong Y (eds) Fauna Sinica, Reptilia, vol 3. (Squamata: Serpentes) Science Press, Bei**g
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genoty** errors in microsatellite data. Mol Ecol Notes 4:535–538
Zhao EM (1998) Elaphe schrenckii. In: Zhao EM (ed) China red data book of endangered animals, Amphibia and Reptilia. Science Press, Bei**g
Zheng XX, Du Y, Lin LH, Lin CX (2014) Development of microsatellite markers in the gray ratsnake Ptyas korros (Colubridae). Conserv Genet Resour. doi:10.1007/s12686-013-0121-
Acknowledgments
The work was carried out in compliance with the current laws of China, and was supported by grants from Natural Science Foundation of China (31272294 and 31200283), the Priority Academic Program Development of Jiangsu Higher Education Institutions (CXLX11_0885), the Jiangsu Provincial Foundation of Natural Science (BK2012849) and the NSFC for Talents Training in Basic Science (J1103507). The authors would like to thank Yan-qing Wu for his help during the research.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Xu, Xy., Fu, Tl., Qu, YF. et al. Isolation and characterization of microsatellite loci in the endangered Amur ratsnake Elaphe schrenckii (Reptilia; Squamata; Colubridae) from China. Conservation Genet Resour 6, 645–646 (2014). https://doi.org/10.1007/s12686-014-0166-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-014-0166-z