Abstract
We characterize fifteen polymorphic microsatellite loci isolated from Ichthyophis bannanicus genomic libraries, which enriched for ATAG-motif microsatellites. The fifteen Loci were screened on a group of 32 individuals from Mengla population. These markers revealed a relatively high degree of genetic diversity (3–13 alleles per locus) and heterozygosity (Ho ranged from 0.036 to 0.935 and He ranged from 0.105 to 0.892). In the fifteen loci, just three showed deviation from Hardy–Weinberg equilibrium (HWE). The high level of polymorphism revealed by these microsatellite markers will prove useful for the study of gene flow, population structure and evolutionary history of Ichthyophis bannanicus.
This is a preview of subscription content, log in via an institution to check access.
References
Fei L, Hu S, Ye C, Huang Y (2006) Fauna Sinica amphibia. Science Press, Bei**g
Freitas PD, Martins DS, Galettijr PM (2008) CID: a rapid and efficient bioinformatic tool for the detection of SSRs from genomic libraries. Mol Ecol Resour 8:107–108
Li G, Meng S, Luo X, Wang H, Bei Y, Zhang B (2010) A preliminary study of Ichthyophis bannanicus on its range, origin and distribution approach by the molecular genetics method. Acta Zootaxonomica Sinica 35(2):338–344
Meng S, Li G (2006) Research advances of Ichthyophis bannanicus. Sichuan J Zool 25(2):423–425
Raymond M, Rousset F (1995) GenePop version 1.2.: population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Sambrook JEF, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
Wen Y (1998) The current status of Ichthyophis bannanicus Yang. Sichuan J Zool 17(2):54
Yang D, Su C (1976) Ichthyophis glutinosus found from **shuangbanna of Yunnan. Acta Zool Sinica 22(1):175
Zane L, Bargelleoni L, Patarenello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16
Acknowledgments
We acknowledge the financial support provided from Natural Science Foundation of Guangxi (No.0832285). We are further grateful to Prof. Qing Chang and his group for their help in laboratory work.
Author information
Authors and Affiliations
Corresponding author
Additional information
Guifen Li and **a Luo are equally contributed to this article.
Rights and permissions
About this article
Cite this article
Li, G., Luo, X., Wang, H. et al. Isolation and characterization of fifteen polymorphic tetranucleotide microsatellites for Ichthyophis bannanicus (Gymnophiona: Ichthyophiidae). Conservation Genet Resour 2 (Suppl 1), 129–131 (2010). https://doi.org/10.1007/s12686-010-9246-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-010-9246-x