Abstract
Nanotechnology is contributed to the techniques, which is well known for the production of unique morphology nanoparticles (NPs) through an environmentally satisfactory method. The present research is designed for the production of gold NPs (AuNPs) using Hippeastrum hybridum (HH) extract and then to test the potential of these AuNPs as ex vivo ant-acetyl cholinesterase (anti-AChE) inhibitor. These AuNPs were analyzed by UV-visible spectroscopy, SEM, FT-IR, EDX, and XRD. FT-IR confirmed the functional groups on the AuNPs surface, XRD report showed the crystal AuNPs of 10.72 nm size, SEM showed irregularly shaped morphology of AuNPs with 30 nm size, and EDX analysis confirmed 48.08% Au signal. A significant anti-AChE potential of 131±0.33 μg IC50 was exhibited by these AuNPs. According to Lineweaver-Burk AuNPs inhibit AChE in the non-competitive route (Km remained unchanged, while Vmax decrease from 1.358 to 0.28). Similarly, AuNPs showed no effect against KIapp, while causing an increase in Vmaxiapp value (11 to 23.35) The Km (Michaelis-Menten kinetic constants), KI (dissociation constant), and Ki (inhibitory constant) were reported to be 0.02 mM, 7.32 μg, and 32 μg. So, it is assumed that HH extract holds the potential for the generation of AuNPs that subsequently proved to be an anti-Alzheimer agent.
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The present study was supported by the higher education commission of Pakistan via project no. 20-2171/NRPU/R&D/HEC/13/5610.
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NS, MA, and NM performed experiments, analyzed data, and wrote the paper. All authors read and approved the final manuscript.
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Sher, N., Ahmed, M. & Mushtaq, N. Plant-Based Synthesis of AuNPs Using Hippeastrum hybridum (L.): Their Ex vivo Anti-acetylcholinesterase Property. BioNanoSci. 13, 1766–1778 (2023). https://doi.org/10.1007/s12668-023-01227-6
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DOI: https://doi.org/10.1007/s12668-023-01227-6