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Recombinant histone H1.3 inhibits orthohantavirus infection in vitro

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Abstract

Histones are proteins participating in DNA packaging. Despite this limited function, histones were shown to have antiviral activity. Histone H1.3 has been shown to inhibit adenovirus infection. Orthohantaviruses are viruses which can cause two diseases: hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). There are no current FDA-approved vaccines or antivirals for hantavirus infection. In this study, we analyzed the effect of recombinant histone H1.3 on Prospect Hill virus (PHV) replication in A549 cells. PHV virus S segment gene and myxovirus resistance protein 1 (MxA), chemokine (C-C motif) ligand 5 (CCL5), and the 10-kDa interferon-inducible protein (IP10) cellular gene expression were evaluated in A549 cells treated with histone H1.3 using qPCR. The expression of PHV virus S segment gene mRNA was significantly decreased in A549 cells when incubated with histone H1.3 compared with PHV controls. CCL5, MxA, and IP10 gene mRNA expression in A549 cells incubated with histone H1.3 before PHV infection was also significantly reduced compared with control PHV only–infected cells. These results suggest that histone H1.3 reduces PHV transduction.

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Funding

This work was funded by the subsidy allocated to KFU for the state assignment in the sphere of scientific activities and by the Russian Government Program of Competitive Growth of KFU.

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Authors and Affiliations

  1. Kazan Federal University, Kazan, Russia

    Daria S. Chulpanova, Valeriya V. Solovyeva, Svetlana F. Khaiboullina & Albert A. Rizvanov

  2. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, The Russian Academy of Sciences, Moscow, Russia

    Daria S. Chulpanova & Valeriya V. Solovyeva

  3. Kazan Scientific-Research Institute of Epidemiology and Microbiology, Kazan, Russia

    Guzel S. Isaeva

  4. Department of Microbiology and Immunology, University of Nevada, Reno School of Medicine, Reno, NV, USA

    Stephen St. Jeor & Svetlana F. Khaiboullina

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  1. Daria S. Chulpanova
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Contributions

Daria S. Chulpanova performed qPCR, carried out the data analyses, and wrote the manuscript; Svetlana F. Khaiboullina and Valeriya V. Solovyeva carried out PHV production and A549 cell infection; Svetlana F. Khaiboullina, Valeriya V. Solovyeva, and Albert A. Rizvanov conceived and designed the study; Guzel S. Isaeva and Stephen St. Jeor edited the manuscript; all authors revised the manuscript and approved the final version.

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Correspondence to Albert A. Rizvanov.

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Chulpanova, D.S., Solovyeva, V.V., Isaeva, G.S. et al. Recombinant histone H1.3 inhibits orthohantavirus infection in vitro. BioNanoSci. 10, 783–791 (2020). https://doi.org/10.1007/s12668-020-00759-5

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