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Abundance of sulphate-reducing bacteria in fish farm sediments along the coast of Japan and South Korea

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Abstract

The abundance of sulphate-reducing bacteria (SRB) was investigated in the coastal marine sediments along the coast of Japan and South Korea. Sediment samples were collected from fish and shellfish farms between 2006 and 2008. As non-fish farming reference sites, sediments were also collected from highly eutrophic bays, a highly sulphidogenic saline lake, and the deep sea. A quantitative real-time PCR analysis that targeted the gene coding for a portion of the α-subunit of dissimilatory sulphite reductase (dsrA) was performed to assess the abundance of the SRB in the sediments. Between 2.8 × 107 and 2.5 × 109 copies of the dsrA gene per gram dry sediment were detected. There was no relationship between dsrA gene copy number and total bacterial count in the sediments, whereas organic matter contents (particulate organic carbon and nitrogen, ignition loss and chemical oxygen demand) and acid-volatile sulphide contents were significantly correlated with the dsrA copy number. The data presented demonstrate that organic enrichment of sediment may influence the abundance of SRB communities in coastal marine sediments and that the cell density of SRB may be used as a biological indicator for assessing pollution levels in sediments of marine fish farms.

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Acknowledgements

We thank N. Nishibori and S. Nishio from Shikoku University; H. Maeda from Mie University; K. Abo, Y. Ishihi and T. Takashi from the National Research Institute of Aquaculture, Fisheries Research Agency; Y. Hayashi from the Kochi Prefectural Fisheries Experiment Station; and I. L. Noh and S. W. Park from Chonnam National University for their help with the field investigations. We also thank fish farm managers I. Hashimoto, S. Nakamu, Y. Shimojo and K. Yamakawa for their field assistance. We are grateful to the captain and the crew of the R/V Tanseimaru for their skillful help in sampling at Tokyo and Sagami Bays. This study was supported in-part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science and the Fisheries Agency of Japan to RK.

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Authors and Affiliations

  1. Department of Marine Bioscience, Fukui Prefectural University, Obama, Fukui, 917-0003, Japan

    Ryuji Kondo, Kotaro Shigematsu, Naoki Kawahara & Takahiko Okamura

  2. Faculty of Marine Technology, College of Fisheries and Ocean Science, Chonnam National University, Dundeok-dong, Yeosu, 550-749, Republic of Korea

    Yang Ho Yoon

  3. Tohoku National Fisheries Research Institute, Fisheries Research Agency, Shiogama, Miyagi, 985-0001, Japan

    Tomoko Sakami

  4. National Research Institute of Aquaculture, Fisheries Research Agency, Minami-Ise, Mie, 561-0193, Japan

    Hisashi Yokoyama

  5. Ehime Prefectural Fisheries Experimental Station, Uwajima, Ehime, 798-0104, Japan

    Yoshitsugu Koizumi

Authors
  1. Ryuji Kondo
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  2. Kotaro Shigematsu
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  3. Naoki Kawahara
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  4. Takahiko Okamura
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  5. Yang Ho Yoon
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  6. Tomoko Sakami
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  7. Hisashi Yokoyama
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  8. Yoshitsugu Koizumi
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Correspondence to Ryuji Kondo.

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Kondo, R., Shigematsu, K., Kawahara, N. et al. Abundance of sulphate-reducing bacteria in fish farm sediments along the coast of Japan and South Korea. Fish Sci 78, 123–131 (2012). https://doi.org/10.1007/s12562-011-0439-3

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  • DOI: https://doi.org/10.1007/s12562-011-0439-3

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