Abstract
Root systems anchor plants to the substrate in addition to transporting water and nutrients, playing a fundamental role in plant survival. The LAZY1 gene mediates gravity signal transduction and participates in root and shoot development and auxin flow in many plants. In this study, a regulator, LsLAZY1, was identified from Leymus secalinus based on previous transcriptome data. The conserved domain and evolutionary relationship were further analyzed comprehensively. The role of LsLAZY1 in root development was investigated by genetic transformation and associated gravity response and phototropism assay. Subcellular localization showed that LsLAZY1 was localized in the nucleus. LsLAZY1 overexpression in Arabidopsis thaliana (Col-0) increased the length of the primary roots (PRs) and the number of lateral roots (LRs) compared to Col-0. Furthermore, 35S:LsLAZY1 transgenic seedlings affected auxin transport and showed a stronger gravitational and phototropic responses. It also promoted auxin accumulation at the root tips. These results indicated that LsLAZY1 affects root development and auxin transport.
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The data that support the results are included within the article and its supplemental files. Other relevant materials are available from the corresponding authors on reasonable request.
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This work was supported by fundings from the National Natural Science Foundation of China (31872415 and 32202484), the Natural Science Foundation of Shandong Province (ZR2022QC008), Scientific Research Leaders Studio of **an (2019GXRC052) and Doctoral Fund project of **an University (XBS2104). The funds played no role in study design, data analysis, and manuscript preparation.
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JL, WH and RSB conceived and designed the experiments. JL, ZW, CS, YN, HL, MK, HC, SW, NY and LH performed the experiments. JL and ZW analyzed the data and wrote the manuscript. WH and RSB revised the manuscript. All authors have read and approved this manuscript.
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Li, J., Wang, Z., Song, C. et al. Identification of LsLAZY1 gene in Leymus secalinus and validation of its function in Arabidopsis thaliana. Physiol Mol Biol Plants 29, 783–790 (2023). https://doi.org/10.1007/s12298-023-01326-4
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DOI: https://doi.org/10.1007/s12298-023-01326-4