Abstract
Fruit morphology and dehiscence-related genes were analyzed in dehiscent N4P and dehiscent P12 Bixa orellana accessions. Fruit architecture (exocarp and pericarp cells, trichomes, vascular bundles, vesicles, and bixin cells) documented by Scanning electron microscopy (SEM) morphology, blue toluidine stain, and phloroglucinol and hydrochloric acid (PHCL) stain was similar in both accessions. Although, the dehiscent zone (DZ) was higher in the indehiscent P12 B. orellana accession, lignification values, obtained by phloroglucinol and hydrochloric acid stain, within the DZ remain was similar in both variants being lower at 34 days after floral anthesis in the dehiscent N4P B. orellana accession. Dehiscence-related genes APETALA (AP2), SHATTERPROOF (SHP), and SPATULA (SPT) were identified on the reported B. orellana transcriptome (SRX1117606). Real-time quantitative polymerase chain reaction primers build by using these genes allow observing a differential expression during six fruit development stages. In both B. orellana accessions, the AP2 transcripts have a reduced expression, whereas the SHP transcripts were significantly higher during the first two days and ten days of development. SPT transcripts show an expression differential between both accessions being significantly higher in the dehiscent N4P, peaking with 9.66% at 42 days after floral anthesis (DAFA) of development. SPT transcription profile suggested that this gene has an important role during the fruit opening in the dehiscent N4P B. orellana accession.
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Acknowledgements
This work was supported by the Consejo Nacional de Ciencia y Tecnología (CONACYT) (CB-2008-01, proyecto No. 220259; Fronteras de la Ciencia No. 2016-01-1716). M.D. Rocio Tamayo-García was financially supported by M.D. and Ph.D scholarship (No. 706229; 8006).
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T-GR and N-ZJA: are co-first authors; these authors contributed equally to this investigation: Conceptualization, Investigation, Data curation, Reviewing, Editing, and Writing. All authors contributed to the Material preparation, data collection and analysis. K-GA, A-EM and G-PLC: Methodology, Investigation, and Data curation. R-MRConceptualization, Resources, Funding acquisition, Reviewing and Writing. R-MR and N-ZJA: Supervising T-GR: work.
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Tamayo-García, R., Narváez-Zapata, J.A., Ku-González, A. et al. Gene expression analysis during the fruit development in dehiscent and indehiscent Bixa orellana L. accessions. Physiol Mol Biol Plants 28, 709–718 (2022). https://doi.org/10.1007/s12298-022-01180-w
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DOI: https://doi.org/10.1007/s12298-022-01180-w