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Boron doped graphdiyne: A metal-free peroxidase mimetic nanozyme for antibacterial application

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Abstract

The abuse of conventional antibiotics leads to increasing bacterial resistance. Nanozyme is a new kind of ultra-efficient and safe nanomaterial with intrinsic enzyme-like activities, showing remarkable potential as a next generation nanobactericide. Graphdiyne (GDY) is a burgeoning two-dimensional (2D) carbon allotrope with intriguing physicochemical properties, holding a great promise as a metal-free nanozyme. In this study, a boron doped GDY nanosheet (B-GDY) was constructed to simulate the performance of peroxidase (POD). By promoting the decomposition of H2O2 to produce reactive oxygen species (ROS), the bactericidal efficacies against both Gram-positive and Gram-negative bacteria were substantially enhanced attributed to the extremely high catalytic activity of B-GDY. In-depth density functional theory (DFT) calculations illuminate that do** of boron can introduce more active B-defect sites as well as lower Gibbs free energy during the H2O2 decomposition reaction. Notably, B-GDY contributes to significant wound healing and excellent biocompatibility, reducing the biological burden. The design of this nanozyme opens a new avenue for the development of alternative antibiotics.

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Acknowledgements

The authors acknowledge Prof. Dongqin Yang (Fudan University) for kindly providing the animal experiments. Animal experiments and care were approved by the Animal Care and Use Committee of Fudan University. This work was supported by the National Natural Science Foundation of China (Nos. 31800800, 21790052, and 21501106), Natural Science Foundation of Shandong Province China (Nos. ZR2019BC101 and ZR2020MB026).

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  1. Xuelong Bi and Qiang Bai contributed equally to this work.

Authors and Affiliations

  1. College of Materials Science and Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Xuelong Bi, Qiang Bai, Fanglin Du, Manhong Liu, Zhiling Zhu & Ning Sui

  2. Center for Nanochemistry, Bei**g Science and Engineering Center for Nanocarbons, National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Bei**g, 100871, China

    Jiaqiang Li, Ning Sui & ** Zhang

  3. College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Xuelong Bi & Lina Wang

  4. Department of Chemistry and Physics, Louisiana State University Shreveport, One University Place, Shreveport, LA, 71115, USA

    William W. Yu

  5. Department of Chemistry, University of Houston, Houston, TX, 77204, USA

    Siheng Li

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Bi, X., Bai, Q., Wang, L. et al. Boron doped graphdiyne: A metal-free peroxidase mimetic nanozyme for antibacterial application. Nano Res. 15, 1446–1454 (2022). https://doi.org/10.1007/s12274-021-3685-4

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