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Electrophysiological properties of spinal wide dynamic range neurons in neuropathic pain rats following spinal nerve ligation

脊神经结扎神经痛大鼠脊髓背角广动力范围神经元的电生理学特性

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Abstract

Objective

The present study aimed to investigate the electrophysiological properties of wide dynamic range (WDR) neurons in spinal dorsal horn of rats with neuropathic pain induced by lumber 5 (L5) spinal nerve ligation (SNL) in a large size of samples.

Methods

Adult Sprague-Dawley rats were divided into normal and SNL groups. Electrophysiological technique was used to record the characteristics of WDR neurons in the spinal dorsal horn.

Results

Compared with the WDR neurons in normal rats, the WDR neurons in SNL rats showed an increase in excitability, manifested by an enlargement of the receptive field size, an increase in the proportion of neurons that exhibited spontaneous activities, decreases in the Cresponse threshold and latency, and an increase in the C-response duration. In addition, the numbers of Aβ- and C-fiberevoked discharges were smaller in SNL rats than in normal rats.

Conclusion

The excitability of spinal WDR neurons increased in rats with neuropathic pain induced by L5 SNL. The increase in excitability of WDR neurons may contribute to the development of neuropathic pain.

摘要

目的

探讨腰5 脊神经结扎(spinal nerve ligation, SNL)后, 大鼠脊髓背角的广动力范围(wide dynamic range, WDR)神经元电生理学特性的改变。

方法

将健康雄性Sprague-Dawley 大鼠分为**常组和SNL 组, 利用细胞外电生理学方法记录脊髓背角的WDR 神经元放电。

结果

与**常大鼠相比, SNL 组大鼠WDR 神经元兴奋性增加, 表现为感受野扩大、 有自发放电的神经元比例增加, 以及 C 纤维诱发放电的阈值降低、 潜伏期缩短、 发放时程增加。 此外, SNL 组大鼠WDR 神经元Aβ 和C 纤维诱发放电数目较**常大鼠降低。

结论

大鼠腰5 脊神经结扎后主要引起WDR 神经元的兴奋性增加。 WDR 神经元的兴奋性增加可能参与神经病理痛的发生。

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Authors and Affiliations

  1. Neuroscience Research Institute, Peking University, Bei**g, 100191, China

    Feng-Yu Liu  (刘风雨), **ao-**u Qu  (曲晓秀), Jie Cai  (蔡捷), Fa-Tian Wang  (王发田), Guo-Gang **ng  (邢国刚) & You Wan  (万有)

  2. Department of Neurobiology, School of Basic Medical Sciences, Peking University, Bei**g, 100191, China

    You Wan  (万有)

  3. Key Laboratory for Neuroscience, the Ministry of Education / the Ministry of Health, Peking University, Bei**g, 100191, China

    You Wan  (万有)

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  1. Feng-Yu Liu  (刘风雨)
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  2. **ao-**u Qu  (曲晓秀)
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  3. Jie Cai  (蔡捷)
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  4. Fa-Tian Wang  (王发田)
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  5. Guo-Gang **ng  (邢国刚)
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  6. You Wan  (万有)
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Correspondence to You Wan  (万有).

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Liu, FY., Qu, XX., Cai, J. et al. Electrophysiological properties of spinal wide dynamic range neurons in neuropathic pain rats following spinal nerve ligation. Neurosci. Bull. 27, 1–8 (2011). https://doi.org/10.1007/s12264-011-1039-z

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  • DOI: https://doi.org/10.1007/s12264-011-1039-z

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