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Multiresistance of Staphylococcus xylosus and Staphylococcus equorum from Slovak Bryndza cheese

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Abstract

Staphylococcus xylosus, Staphylococcus equorum, and Staphylococcus epidermidis strains were isolated from Bryndza cheese and identified using PCR method. The antimicrobial susceptibility of these strains was assessed using disc diffusion method and broth microdilution method. The highest percentage of resistance was detected for ampicillin and oxacillin, and in contrary, isolates were susceptible or intermediate resistant to ciprofloxacin and chloramphenicol. Fourteen of the S. xylosus isolates (45 %) and eleven of the S. equorum isolates (41 %) exhibited multidrug resistance. None of the S. epidermidis isolate was multiresistant. The phenotypic resistance to oxacillin was verified by PCR amplification of the gene mecA.

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Acknowledgments

This contribution is the result of the following project implementation: ITMS 26240120027 supported by the Research & Development Operational Programme, funded by the ERDF, and was supported by project VEGA 1/0892/11.

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Authors and Affiliations

  1. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynská dolina, 842 15, Bratislava, Slovakia

    Mária Mikulášová, Jana Valáriková, Romana Chovanová & Anna Belicová

  2. Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina, 842 15, Bratislava, Slovakia

    Roman Dušinský

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  1. Mária Mikulášová
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  2. Jana Valáriková
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  3. Roman Dušinský
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  5. Anna Belicová
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Correspondence to Anna Belicová.

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Mikulášová, M., Valáriková, J., Dušinský, R. et al. Multiresistance of Staphylococcus xylosus and Staphylococcus equorum from Slovak Bryndza cheese. Folia Microbiol 59, 223–227 (2014). https://doi.org/10.1007/s12223-013-0286-y

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  • DOI: https://doi.org/10.1007/s12223-013-0286-y

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