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Membrane proteins and squalene-hydrosqualene profile in methanoarchaeon Methanothermobacter thermautotrophicus resistant to N,N′-dicyclohexylcarbodiimide

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Abstract

The biochemical basis of a defective bioenergetic system was attempted to be determined in N,N′-dicyclohexylcarbodiimide (DCCD)-resistant mutant of Methanothermobacter thermautotrophicus. Components participating in the maintenance of methanoarchaeal membrane structure and function, such as the composition of the mixture of squalene and its hydrosqualene derivatives and also properties of membrane-associated proteins were compared in wild-type and mutant cells. The impairment of the bioenergetic system in DCCD-resistant mutant was detectable in the membrane-protein profile; it was also accompanied by changes in proportions of squalene-hydrosqualenes.

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Abbreviations

DCCD:

N,N′-dicyclohexylcarbodiimide

MS:

mass spectrometry

SDS-PAGE:

sodium dodecylsulfate polyacrylamide gel electrophoresis

TCS:

3,5,3′,4′-tetrachlorosalicylanilide

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Authors and Affiliations

  1. Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, 900 28, Ivanka pri Dunaji, Slovakia

    Z. Nováková, S. Šurín, A. Majerník & P. Šmigáň

  2. Institute of Chemistry, Faculty of Science, Comenius University, 845 45, Bratislava, Slovakia

    J. Blaško

Authors
  1. Z. Nováková
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  2. S. Šurín
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  3. J. Blaško
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  4. A. Majerník
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  5. P. Šmigáň
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Corresponding author

Correspondence to P. Šmigáň.

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These authors contributed equally to this work.

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Nováková, Z., Šurín, S., Blaško, J. et al. Membrane proteins and squalene-hydrosqualene profile in methanoarchaeon Methanothermobacter thermautotrophicus resistant to N,N′-dicyclohexylcarbodiimide. Folia Microbiol 53, 237–240 (2008). https://doi.org/10.1007/s12223-008-0034-x

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  • DOI: https://doi.org/10.1007/s12223-008-0034-x

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