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Isolation and Characterization of Trichoderma spp. for Antagonistic Activity Against Root Rot and Foliar Pathogens

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Abstract

Trichoderma, soil-borne filamentous fungi, are capable of parasitising several plant pathogenic fungi. Twelve isolates of Trichoderma spp. isolated from different locations of South Andaman were characterized for their cultural, morphological and antagonistic activity against soil borne and foliar borne pathogens. The sequencing of these isolates showed seven different species. The isolates revealed differential reaction patterns against the test pathogens viz., Sclerotium rolfsii, Colletotrichum gloeosporioides and C. capsici. However, the isolates, TND1, TWN1, TWC1, TGD1 and TSD1 were most effective in percentage inhibition of mycelial growth of test pathogens. Significant chitinase and β-1,3-glucanase activities of all Trichoderma isolates has been recorded in growth medium. T. viride was found with highest chitinase whereas T. harzianum was recorded with highest β-1,3-glucanase activities.

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Acknowledgments

This research was supported by grants from the project “Application of Microorganisms in Agriculture and Allied Sectors” of the NBAIM-ICAR, UP, Mau, India. We thank the Head Division of Field Crops, CARI for constant support and encouragements during the course of study.

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Authors and Affiliations

  1. Division of Field Crops, Central Agricultural Research Institute, Port Blair, 744 105, Andaman and Nicobar Islands, India

    Krishna Kumar, N. Amaresan, S. Bhagat, K. Madhuri & R. C. Srivastava

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  1. Krishna Kumar
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  2. N. Amaresan
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  3. S. Bhagat
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  4. K. Madhuri
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  5. R. C. Srivastava
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Correspondence to Krishna Kumar.

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Kumar, K., Amaresan, N., Bhagat, S. et al. Isolation and Characterization of Trichoderma spp. for Antagonistic Activity Against Root Rot and Foliar Pathogens. Indian J Microbiol 52, 137–144 (2012). https://doi.org/10.1007/s12088-011-0205-3

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  • DOI: https://doi.org/10.1007/s12088-011-0205-3

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