Abstract
Low-molecular-weight glutenin subunits (LMW-GSs) are one of the important factors for the dough processing quality. In this study, a novel LMW-GS, designated LMW-N13, from the wheat relative species Aegilops uniaristata PI 554421 was cloned and characterized. Unlike previously published LMW-GSs, LMW-N13 has a large molecular weight and is the largest LMW-GS published thus far. Sequence alignments demonstrated that LMW-N13 is a LMW-i-type subunit but contains nine cysteine residues which is one more than typical LMW-i-type subunits. In addition, four insertions are present in the repetitive domain that resulted in the large molecular weight. In vitro analysis showed that LMW-N13 could improve the dough quality of different base flours.
This is a preview of subscription content, log in via an institution to check access.
References
An X., Zhang Q., Yan Y., Li Q., Zhang Y., Wang A. et al. 2006 Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn (Triticum monococcum L.). Theor. Appl. Genet. 113, 383–395.
Chen F. G., Zhao F., Liu R. K. and **a G. M. 2011 Functional properties of two low-molecular-weight glutenin subunits carrying additional cysteine residues from hybrid introgression line II-12 derived from Triticum aestivum and Agropyron elongatum. Food Chem. 127, 1773–1776.
D’Ovidio R. and Masci S. 2004 The low-molecular-weight glutenin subunits of wheat gluten. J. Cereal Sci. 39, 321–339.
Du X. Y. and Zhang X. C. 2017 Molecular cloning and functional characterization of two novel high molecular weight glutenin subunit genes in Aegilops markgrafii. J. Genet. 96, 563–570.
Feeney K. A., Tatham A. S., Gilbert S. M., Fido R. J., Halford N. G. and Shewry P. R. 2001 Synthesis, expression and characterisation of peptides comprised of perfect repeat motifs based on a wheat seed storage protein. Biochem. Biophys. Acta 1546, 346–355.
Ikeda T. M., Nagamine T., Fukuoka H. and Yano H. 2002 Identification of new low-molecular-weight glutenin subunit genes in wheat. Theor. Appl. Genet. 104, 680–687.
Li X. H., Wang A. L., **ao Y. H., Yan Y. M., He Z. H., Appels R. et al. 2008 Cloning and characterization of a novel low molecular weight glutenin subunit gene at the Glu-A3 locus from wild emmer wheat (Triticum turgidum L. var. dicoccoides). Euphytica 159, 181–190.
Liu C. Y. and Shepherd K. W. 1995 Inheritance of B subunits of glutenin and \({\upomega }\)- and \({\upgamma }\)-gliadins in tetraploid wheats. Theor. Appl. Genet. 90, 1149–1157.
Ma W., Appels R., Bekes F., Larroque O., Morell M. K. and Gale K. R. 2005 Genetic characterization of dough rheological properties in a wheat doubled haploid population: additive genetic effects and epistatic interactions. Theor. Appl. Genet. 111, 410–422.
Ma C. Y., Yang Y., Li X. H., Ge P., Guo G. F., Subburaj S. et al. 2013 Molecular cloning and characterization of six novel HMW-GS genes from Aegilops speltoides and Aegilops kotschyi. Plant Breed. 132, 248–239.
Masci S., D’ Ovidio R., Lafiandra D. and Kasarda D. D. 1998 Characterization of a low-molecular-weight glutenin subunit gene from bread wheat and the corresponding protein that represents a major subunit of the glutenin polymer. Plant Physiol. 118, 1147–1158.
Masci S., D’ Ovidio R., Lafiandra D. and Kasarda D. D. 2000 A 1B coded low-molecular-weight glutenin subunit associated with quality in durum wheats show strong similarity to subunits present in some bread wheat cultivars. Theor. Appl. Genet. 100, 396–400.
Pei Y. H., Wang A. L., An X. L., Li X. H., Zhang Y. Z., Huang X. Q. et al. 2006 Characterization and comparative analysis of three low molecular weight glutenin C-subunit genes isolated from Aegilops tauschii. Can. J. Plant Sci. 87, 273–280.
Pogna P. E., Autran J. C., Mellini F., Lafiandra D. and Feillet P. 1990 Chromosome 1B-encoded gliadins and glutenin subunits in durum wheat: genetics and relationship to gluten strength. J. Cereal Sci. 11, 15–34.
Shewry P. R., Halford N. G. and Tatham A. S. 1992 High molecular weight subunits of wheat glutenin. J. Cereal Sci. 15, 105–120.
Sreeramulu G. and Singh N. K. 1997 Genetic and biochemical characterization of novel low molecular weight glutenin subunits in wheat. Genome 40, 41–48.
Takata K., Yamauchi H., Lriki N. and Kuwabara T. 1999 Prediction of bread-making quality by prolonged swelling SDS-sedimentation test. Breed. Sci. 49, 221–223.
Wang S. L., Li X. H., Wang K., Wang X. Z., Li S. S., Zhang Y. Z. et al. 2011 Phylogenetic analysis of C, M, N, and U genomes and their relationships with Triticum and other related genomes as revealed by LMW-GS genes at Glu-3 loci. Genome 54, 273–284.
Zhang X. F., ** H., Zhang Y., Liu D. C., Li G. Y., **a X. C. et al. 2012 Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat. BMC Plant Biol. 12, 243.
Zhen S. M., Han C. X., Ma C. Y., Gu A. Q., Zhang M., Shen X. X. et al. 2014 Deletion of the low-molecular-weight glutenin subunits allele Glu-A3a of wheat (Triticum aestivum L.) significantly reduces dough strength and breadmaking quality. BMC Plant Biol. 14, 367.
Acknowledgements
We thank Prof. Lihui Li for providing the Ae. uniaristata PI 554421. This work was supported by the National Natural Science Foundation of China (31471488 and 31520103911), the Key Technology Projects of Independent Innovation of Shandong Province, China (2014GJJS0201-3-1) and Shandong Agricultural Seed Improvement Project.
Author information
Authors and Affiliations
Corresponding authors
Additional information
Corresponding editor: U. C. Lavania
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Tang, H., Du, X., Wang, H. et al. Cloning and characterization of a novel low-molecular-weight glutenin subunit gene with an unusual molecular structure of Aegilops uniaristata. J Genet 97, 1463–1468 (2018). https://doi.org/10.1007/s12041-018-1020-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12041-018-1020-y