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Apoptosis induced by (di-isopropyloxyphoryl-Trp)2-Lys-OCH3 in K562 and HeLa cells

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Abstract

According to the method used in our laboratory, our group synthesized (DIPP-Trp)2-Lys-OCH3. It inhibited the proliferation of K562 and HeLa cells in a dose-and time-dependent manner with an IC50 of 15.12 and 42.23 µM, respectively. (DIPP-Trp)2-Lys-OCH3 induced a dose-dependent increase of the G2/M cell population in K562 cells, and S cell population in HeLa cells; the sub-G0 population increased dramatically in both cell lines as seen by PI staining experiments using a FACS Calibur Flow cytometer (BeckmanCoulter, USA). Phosphatidylserine could significantly translocate to the surface of the membrane in (DIPP-Trp)2-Lys-OCH3-treated K562 and HeLa cells. The increase of an early apoptotic population was observed in a dose-dependent manner by both annexin-FITC and PI staining. It was concluded that (DIPP-Trp)2-Lys-OCH3 not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle. It may have arrested the K562 and HeLa cells in the G2/M, S phases, respectively. The apoptotic pathway was pulsed at this point, resulting in the treated cells entering into programmed cell death. (DIPP-Trp)2-Lys-OCH3 is a potential anticancer drug that intervenes in the signalling pathway.

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Abbreviations

Apaf-1:

apoptotic protease activating factor-1

(DIPP-Trp)2-Lys-OCH3 :

(di-isopropyloxyphoryl-Trp)2-Lys-OCH3

FBS:

foetal bovine serum

IC50 :

inhibitory concentration 50%

IR%:

inhibition rate

MTT:

3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide

PI:

propidium iodide

PS:

phosphatidylserine

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Authors and Affiliations

  1. The Key Laboratory of Chemical Biology, Guangdong Province, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China

    Feng Liu, Shi-Ying Liu, Zhen-Hua **e & Yu-Yang Jiang

  2. Department of Biological Sciences and Biotechnology, Tsinghua University, Bei**g, 100084, China

    Feng Liu & Guo-** Cai

  3. College of Bioengineering, Henan University of Technology, Zhengzhou, 450052, China

    ** Xu

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  1. Feng Liu
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  2. Shi-Ying Liu
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  3. ** Xu
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  4. Zhen-Hua **e
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  5. Guo-** Cai
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  6. Yu-Yang Jiang
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Correspondence to Yu-Yang Jiang.

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Liu, F., Liu, SY., Xu, P. et al. Apoptosis induced by (di-isopropyloxyphoryl-Trp)2-Lys-OCH3 in K562 and HeLa cells. J Biosci 33, 55–62 (2008). https://doi.org/10.1007/s12038-008-0001-3

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