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Protective Effect of Agaricus blazei Polysaccharide Against Cadmium-Induced Damage on the Testis of Chicken

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Abstract

Cadmium (Cd) exposure can cause reproductive toxicity through oxidative stress and inflammatory response. A polysaccharide extract of the edible mushroom Agaricus blazei Murill has been isolated and exhibits antioxidant activity and immunoregulatory effect. The aim of this study was to investigate the protective role of Agaricus blazei polysaccharide (ABP) against Cd-induced damage in chicken testis through enhancing antioxidant activity and alleviating inflammatory response. One hundred twenty healthy 7-day-old Hy-Line male chickens (Harbin, China) were randomly divided into four groups, and each group consisted of 30 chickens: Normal control was fed daily with full feed and 0.2 mL distilled water per day via oral gavage; Cd-treated group was fed daily with full feed that contained 140 mg/kg CdCl2 and 0.2 mL distilled water per day by gavage; Polysaccharide-treated group was fed daily with full feed with 0.2 mL ABP(30 mg/ml) solution per day via oral gavage; Cd/polysaccharide-treated group was fed daily with full feed containing 140 mg/kg CdCl2 and 0.2 mL ABP(30 mg/ml) solution per day by gavage. On the 20, 40, and 60 days, the testis was immediately removed. The contents of Cd in the testis, activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), malondialdehyde (MDA) production, messenger RNA (m RNA) levels of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6), protein expressions of heat shock proteins (HSPs) (HSP60, HSP70, and HSP90), and the histopathological changes of the testis were determined. The results indicated that ABP improved Cd-caused testicular tissue damage by increasing the SOD and GSH-Px activities: decreasing the Cd accumulation and MDA content, mRNA levels of TNF-α, IL-1β, and IL-6, and protein expressions of HSP60, HSP70, and HSP90. Results suggest that ABP for the mitigation of damage induced by cadmium in chicken testis through enhancing antioxidant activity and alleviating Inflammatory response.

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Acknowledgements

We thank the members of the Traditional Chinese Veterinary Medicine Laboratory in the College of Veterinary Medicine, Northeast Agricultural University.

Funding

This work was supported by the National Science Foundation of China (Grant No. 31272533).

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  1. Yangyang Song and Ruili Zhang contributed equally to this work.

Authors and Affiliations

  1. Department of Clinical Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China

    Yangyang Song, Ruili Zhang, Hongmei Wang, Yan Yan & Ge Ming

  2. Heilongjiang Key Laboratory for Animal Diseases, Harbin, 150030, People’s Republic of China

    Yangyang Song, Ruili Zhang, Hongmei Wang, Yan Yan & Ge Ming

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  1. Yangyang Song
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Correspondence to Ge Ming.

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All procedures in this study have been approved by the Institutional Animal Care and Use Committee of Northeast Agricultural University in China.

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Song, Y., Zhang, R., Wang, H. et al. Protective Effect of Agaricus blazei Polysaccharide Against Cadmium-Induced Damage on the Testis of Chicken. Biol Trace Elem Res 184, 491–500 (2018). https://doi.org/10.1007/s12011-017-1196-7

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