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Optimization of High Solids Dilute Acid Hydrolysis of Spent Coffee Ground at Mild Temperature for Enzymatic Saccharification and Microbial Oil Fermentation

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Abstract

Soluble coffee, being one of the world’s most popular consuming drinks, produces a considerable amount of spent coffee ground (SCG) along with its production. The SCG could function as a potential lignocellulosic feedstock for production of bioproducts. The objective of this study is to investigate the possible optimal condition of dilute acid hydrolysis (DAH) at high solids and mild temperature condition to release the reducing sugars from SCG. The optimal condition was found to be 5.3 % (w/w) sulfuric acid concentration and 118 min reaction time. Under the optimal condition, the mean yield of reducing sugars from enzymatic saccharification of defatted SCG acid hydrolysate was 563 mg/g. The SCG hydrolysate was then successfully applied to culture Lipomyces starkeyi for microbial oil fermentation without showing any inhibition. The results suggested that dilute acid hydrolysis followed by enzymatic saccharification has the great potential to convert SCG carbohydrates to reducing sugars. This study is useful for the further develo** of biorefinery using SCG as feedstock at a large scale.

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Acknowledgments

This work was supported by grants from the National Science Council and Ministry of Science and Technology, Taiwan (NSC102-2622-E-037-002, and MOST 103-2221-E-224-085).

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Authors and Affiliations

  1. Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan

    Hui-Min David Wang

  2. Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Douliou, Yunlin, 64002, Taiwan

    Yu-Shen Cheng, Chi-Hao Huang & Chia-Wei Huang

Authors
  1. Hui-Min David Wang
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  2. Yu-Shen Cheng
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  3. Chi-Hao Huang
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  4. Chia-Wei Huang
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Correspondence to Yu-Shen Cheng.

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Figure S1

SEM images of untreated SCG (a), diluted acid treated SCG (b) and diluted acid treated followed by enzymatic treated SCG (c) (DOCX 1.31 mb)

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Wang, HM.D., Cheng, YS., Huang, CH. et al. Optimization of High Solids Dilute Acid Hydrolysis of Spent Coffee Ground at Mild Temperature for Enzymatic Saccharification and Microbial Oil Fermentation. Appl Biochem Biotechnol 180, 753–765 (2016). https://doi.org/10.1007/s12010-016-2130-8

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  • DOI: https://doi.org/10.1007/s12010-016-2130-8

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