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Biohydrogen production from glycerol by novel Clostridium sp. SH25 and its application to biohydrogen car operation

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Abstract

Biohydrogen is a clean and efficient source of energy produced easily by anaerobic systems. Therefore, the discovery of novel and efficient production methods and utilization of inexpensive starting material are crucial for economical biohydrogen production. In this study, novel hydrogen producing bacterial strain Clostridium sp. SH25 was screened from the anaerobic sludge obtained from a water treatment plant, which showed a higher hydrogen-producing activity on glycerol than other strains. The effective hydrogen production was evaluated under varying anaerobic culture conditions, and the optimum temperature, initial pH, additional NaCl concentration, and inoculum size were 37 °C, 6.0, 0%, and 10% (v/v), respectively. The cumulative hydrogen production volume from crude glycerol was 24.30±1.07 ml after 36 h. To test the practical application of biohydrogen, a 20 ml culture of Clostridium sp. SH25 was incubated for 12 h and directly applied to a small hydrogen car unit operated for 19.05±0.33 s with 8.37 ± 0.21 m displacement. Overall, identification of the efficient Clostridium sp. SH25 strain resulted in the production of a large amount of biohydrogen, which further supported the operation of a small hydrogen car. This implied a possible application of biosystems in biohydrogen production.

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Acknowledgements

This study was supported by the Research Program to solve social issues with the National Research Foundation of Korea (NRF), funded by the Ministry of Science and ICT [grant number 2017 M3A9E4077234], National Research Foundation of Korea (NRF) [grant numbers NRF-2022R1A2C2003138 and NRF-2019M3E6 A1103979]. This study was also supported by the R&D Program of MOTIE/KEIT [grant number 20016324].

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Authors and Affiliations

  1. Department of Biological Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwang**-gu, Seoul, 05029, Korea

    Sang Hyun Kim, Hyun Joong Kim, Shashi Kant Bhatia, Ranjit Gurav & Yung-Hun Yang

  2. Green & Sustainable Materials R&D Department, Research Institute of Clean Manufacturing System, Korea Institute of Industrial Technology (KITECH), 89, Yangdaegiro-gil, Ipjang-myeon, Seobuk-gu, Cheonan-si, Chungcheongnam-do, 31056, Korea

    Jong-Min Jeon & Jeong-Jun Yoon

  3. School of Civil and Environmental Engineering, Yonsei University, 50, Yonsei-ro, Seodaemun-gu, Seoul, 03722, Korea

    Sang-Hyoun Kim

  4. Biotechnology Process Engineering Center, Korea Research Institute Bioscience Biotechnology (KRIBB), 125, Gwahak-ro, Yuseong-gu, Daejeon, 34141, Korea

    Jungoh Ahn

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  1. Sang Hyun Kim
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Correspondence to Yung-Hun Yang.

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Kim, S.H., Kim, H.J., Bhatia, S.K. et al. Biohydrogen production from glycerol by novel Clostridium sp. SH25 and its application to biohydrogen car operation. Korean J. Chem. Eng. 39, 2156–2164 (2022). https://doi.org/10.1007/s11814-022-1146-8

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