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Concentrated and gastight sampling technique of deepsea microplankton

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Abstract

A new sampling method of deepsea microplankton with function of in-situ concentrated sampling and gastight sampling was proposed. In-situ concentrated sampling technique was realized as follows: a microplankton membrane was used as filtration membrane, and a deepsea pump was used to pump seawater; the microplankton was captured and the density of microplankton was increased when seawater flow through the filtration membrane. Gastight sampling technique was realized as follows: a precharged accumulator was used as pressure compensator. During the process of lifting the sampler, the accumulator compensated the pressure drop continuously. The laboratory experimental results show that with in-situ concentrated sampling technique, in-situ concentrated sampling can be realized and the maximum concentration ratio reaches up to 500. With pressure compensation technique based on accumulator, gastight sampling can be realized. When sampling at 6 km and the precharge pressure of accumulator is 18 MPa, pressure drop of the sample is less than 2% compared with its original pressure. Deepsea experiment (at 1.9 km) results show that the sampler can realize in-situ concentrated sampling and gastight sampling.

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Correspondence to Huang Zhong-hua  (黄中华).

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Foundation item: Project(DY105-03-01-10) supported by China Ocean Mineral Resources Research and Development Association; Project (1343-75221) supported by Central South University

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Huang, Zh., Liu, Sj., **, B. et al. Concentrated and gastight sampling technique of deepsea microplankton. J. Cent. South Univ. Technol. 14, 820–825 (2007). https://doi.org/10.1007/s11771-007-0156-3

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  • DOI: https://doi.org/10.1007/s11771-007-0156-3

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