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Ameliorative effects of standardized extract of Tamarix stricta Boiss. on acetic acid-induced colitis via modulating nitrergic pathways

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Abstract

Inflammatory bowel disease (IBD) is a chronic recurrent disease with no certain treatment. Tamarisk is a medicinal plant mentioned in Persian medicine as an effective treatment for gastrointestinal bleeding. Current study aimed to investigate the ameliorative effect of Tamarix stricta Boiss. on acetic acid-induced colitis in rats and the role of nitrergic system. The hydro-ethanolic extract was analyzed via liquid chromatography–mass spectrometry. Gallic acid and quercetin were quantified with high-performance liquid chromatography. Following induction of colitis with acetic acid, T. stricta extract (50, 100, 150, and 200 mg/kg) was orally administered for two successive days. The involvement of nitrergic system was examined, using L-NG-Nitro Arginine Methyl Ester (L-NAME) and aminoguanidine as nitric oxide synthase (NOS) inhibitors. Finally, macroscopic and microscopic analyses were performed. Tissue concentrations of inflammatory mediators including interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and myeloperoxidase (MPO) were assessed. T. stricta, especially at 200 mg/kg dose, could significantly improve colitis and downregulate the inflammatory factors including TNF-α, IL-1β, and MPO (P <0.001). Concomitant use of NOS inhibitors with 200 mg/kg of the plant extract reversed the observed positive effects, which demonstrates the protective role of NO in IBD pathogenesis as a probable mechanism for T. stricta in healing process of colitis. The positive effect of T. stricta on colitis might be attributed to anti-inflammatory properties of its polyphenolic components, mainly through modulation of the nitrergic system.

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Abbreviations

ANOVA:

analysis of variances

IBD:

inflammatory bowel diseases

UC:

ulcerative colitis

CD:

Crohn’s disease

IL:

interleukin

TNF:

tumor necrosis factor

TGF:

transforming growth factor

IFN:

interferon

NF-κB:

nuclear factor-kappa B

NO:

nitric oxide

iNOS:

inducible nitric oxide synthase

eNOS:

endothelial nitric oxide synthase

HPLC:

high-performance liquid chromatography

LC-MS:

liquid chromatography-mass spectrometry

ip:

intraperitoneal

L- NAME:

L-NG-Nitro Arginine Methyl Ester

GI:

gastrointestinal tract

MPO:

myeloperoxidase

T-50-200:

Tamarix extract with the doses of 50-200 mg/kg

SEM:

standard error of mean

SPSS:

statistical package for the social sciences

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Funding

This study is partially supported by Tehran University of Medical Sciences (grant No. 97-03-86-40737).

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Authors and Affiliations

  1. Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, P. O. Box: 19419-33111, No. 99, Yakhchal, Gholhak, Shariati St, Tehran, Iran

    Negar Ghanaatian & Amir Hossein Abdolghaffari

  2. Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran

    Saeideh Momtaz & Amir Hossein Abdolghaffari

  3. Toxicology and Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), The Institute of Pharmaceutical Sciences (TIPS), and Department of Toxicology and Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, 1417614411, Iran

    Saeideh Momtaz, Maryam Baeeri & Amir Hossein Abdolghaffari

  4. Faculty of Pharmacy, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Poursina Avenue, PO Box. 14155-6451, Tehran, Iran

    Saeideh Momtaz & Amir Hossein Abdolghaffari

  5. Gastrointestinal Pharmacology Interest Group (GPIG), Universal Scientific Education and Research Network (USERN), Tehran, Iran

    Saeideh Momtaz & Amir Hossein Abdolghaffari

  6. Department of Traditional Pharmacy, School of Persian Medicine, Tehran University of Medical Sciences, No. 27, North Sarparast, West Taleqani, Felestin Sq., P.O. box: 1417653761, Tehran, Iran

    Roodabeh Bahramsoltani & Roja Rahimi

  7. Phytopharmacology Interest Group (PPIG), Universal Scientific Education and Research Network (USERN), Tehran, Iran

    Roodabeh Bahramsoltani

  8. Evidence‑Based Evaluation of Cost‑Effectiveness and Clinical Outcomes, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran

    Roja Rahimi

Authors
  1. Negar Ghanaatian
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  2. Saeideh Momtaz
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  3. Roodabeh Bahramsoltani
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  4. Maryam Baeeri
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  5. Amir Hossein Abdolghaffari
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  6. Roja Rahimi
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Contributions

RR & AHA designed the study, confirmed the protocol, and edited the manuscript. SM & MB participated in mechanistic studies, data analysis and edited the manuscript. RB participated in data analysis, phytochemical assessments, and edited the manuscript. NG performed the animal study and wrote the manuscript.

Corresponding authors

Correspondence to Amir Hossein Abdolghaffari or Roja Rahimi.

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The authors declare that they have no competing of interest.

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IR.TUMS.VCR.REC.1397.768.

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Ghanaatian, N., Momtaz, S., Bahramsoltani, R. et al. Ameliorative effects of standardized extract of Tamarix stricta Boiss. on acetic acid-induced colitis via modulating nitrergic pathways. Biologia 77, 791–801 (2022). https://doi.org/10.1007/s11756-021-00975-8

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