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Proteomic identification of lipid-bodies-associated proteins in maize seeds

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Abstract

Seeds store lipids in the form of lipid bodies (LBs) for germination and early seedling growth. LBs can be easily isolated by the established floating-extraction method from oleaginous seeds containing a large quantity of LBs. Compared to oleaginous seeds, maize and other cereal seeds contain a small quantity of LBs, so it is difficult to isolate a sufficient quantity of LBs from their embryos for 2DE-based proteomic analysis. At present, only a limited number of LBs-associated proteins in maize embryos have been identified. We here reported a modified floating-extraction method using polyvinylidene difluoride disc to collect floating LBs from maize embryo extracts. The LBs-associated proteins were resolved with two-dimensional electrophoresis and identified with mass spectrometry. As a result, several well-known LBs proteins were identified in the purified LBs fraction, such as oleosin, caleosin, and steroleosin. We also identified another two LBs proteins, corticosteroid 11-β-dehydrogenase 1 and 11-β-hydroxysteroid dehydrogenase-like 5. In particular, steroleosin, corticosteroid 11-β-dehydrogenase 1, 11-β-hydroxysteroid dehydrogenase-like, and hydroxyproline-rich glycoprotein were found as the most abundant protein components in maize LBs. The data set of maize LBs subproteome would provide insights into functional research of LBs-associated proteins during seed development and germination. Additionally, the protocol developed here is expected to be applicable for isolating LBs in other seeds or tissues containing a low quantity of LBs.

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Acknowledgements

This work was supported by the Program for Innovative Research Team (in Science and Technology) in University of Henan Province (15IRTSTHN015).

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  1. State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, China

    Cui Du, Aimei Liu, Liangjie Niu, Di Cao, Hui Liu, **aolin Wu & Wei Wang

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  1. Cui Du
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Correspondence to Hui Liu or Wei Wang.

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Du, C., Liu, A., Niu, L. et al. Proteomic identification of lipid-bodies-associated proteins in maize seeds. Acta Physiol Plant 41, 70 (2019). https://doi.org/10.1007/s11738-019-2854-5

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