Abstract
In this research, the antioxidant properties of the hydrolyzed peptide of chlorella vulgaris (CVPH) with molecular weights (MW) of 3, 5, and 10 were kDa determined by using alcalase and flavourzyme enzyme, and cakes oil cake containing CVPH with a concentration of 0, 0.5 and 1% were produced and moisture, color, textural, chemical (peroxide value, acidity), microbial (Total viable count, yeast and mold) and sensory attributes (color, smell and overall acceptance) of them were evaluated during 30 days of storage at 25 °C. The results related to the characteristics of the bioactive peptide showed that the CVPH had high essential amino acids and CVPH by alcalase with a MW of 3 kDa s had the highest antioxidant activity, and this peptide was used in subsequent experiments. The results of the cake tests showed that the addition of CPVH increased the moisture content and decreased the color index of the cake, as well as CPVH reduced the oxidative and microbial spoilage of the cake during the storage period, and these treatments had a chemical and sensory permissible range until the end of the storage period. The sensory score of the treatment containing CPVH at 0.5% was higher than the treatment containing CPVH at 1%. Therefore, the use of CPVH as a natural preservative can increase the shelf life of the functional oil cake. Considering the relatively similar effect of 0.5% and 1% concentrations, as well as economic efficiency and better sensory characteristics, 0.5% can be the optimal concentration.
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Notes
Dichloran-Rose Bengal Chloramphenicol Agar.
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Taghdiri, S., Emtyazjoo, M., Azizi, M.H. et al. The effect of hydrolyzed protein obtained from Chlorella vulgaris on the shelf life and quality of oil cake during the storage period. Food Measure 18, 582–592 (2024). https://doi.org/10.1007/s11694-023-02186-y
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DOI: https://doi.org/10.1007/s11694-023-02186-y