Abstract
Objective: To analyze the changes of gene expression in phenylbutyrate induced differentiation of glioma cells. Methods: The expression levels of 14000 genes in glioma cells before and after inducement with sodium phenylbutyrate for 2 h or 6 days were evaluated by cDNA array technique and proved by multi-dot blotting. Results: expression of 98 genes in glioma cells showed changes after the inducement. Some genes involved in transcription and translation and some oncogenes are down-regulated, while some gene involved in differentiation or apoptosis are up-regulated. 18 unknown expression sequencing tag (EST) changed too. Conclusion: A gene expression profile associated with differentiation of glioma cells was established.
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Foundation item: This work was supported by the National Natural Science Foundation of China (No. 39870826)
Biography: SUN Li-jun (1974–), male, doctor of medicine, Tian** Huanhu Hospital, majors in neurosurgery.
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Sun, Lj., Huang, Q., Lan, Q. et al. Gene expression profiling of phenylbutyrate induced differentiation of glioma cells by cDNA array. Chin. J. Cancer Res. 15, 38–42 (2003). https://doi.org/10.1007/s11670-003-0009-z
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DOI: https://doi.org/10.1007/s11670-003-0009-z