Abstract
The effects of Timentin and cefotaxime (Cef) on shoot regeneration of the London plane tree (Platanus acerifolia Willd.) and their use for the suppression of Agrobacterium tumefaciens in Agrobacterium-mediated genetic transformation were compared. Shoot regeneration was significantly reduced on the media with Cef at concentrations from 100 to 500 mg·L−1. Timentin showed negative effect on plant regeneration at concentrations of 100 and 500 mg·L−1; however, 300 mg·L−1 Timentin was shown to facilitate shoot regeneration significantly and the regeneration frequency increased from 64% (control) to 88%. Effective suppression of A. tumefaciens could be obtained with 500 mg·L−1 Cef, but plant regeneration was completely inhibited at this level. The A. tumefaciens on infected P. acerifolia leaf tissues was visually undetectable after three subcultures on a medium with 300 mg·L−1 Timentin. Considering the effect of Cef and Timentin on plant regeneration and suppression of Agrobacteria, Timentin at 300 mg·L−1 is the preferred application in A. tumefaciens-mediated transformation of P. acerifolia.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Buckley P M, Reed B M. 1994. Antibiotic susceptibility of plant-associated bacteria. HortScience, 29: 434
Cheng Z M, Schnurr J A, Kapaun J A. 1998. Timentin as an alternative antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation. Plant Cell Rep., 17: 646–649
Colby S M, Juncosa A M, Meredith C P. 1991. Cellular differences in Agrobacterium susceptibility and regeneration capability restrict the development of transgenic grapevines. J. Am. Soc. Hortic. Sci., 116: 356–361
Costa M G C, Nogueira F T S, Figueira M L, Otoni W C, Brommonschenkel S H, Cecon P R. 2000. Influence of the antibiotic Timentin on plant regeneration of tomato (Lycopersicon esculentum Mill.) cultivars. Plant Cell Rep., 19: 327–332
De Bondt A, Eggermont K, Druart P, De Vil M, Goderis I, Vanderleyden J, Broekaert W F. 1994. Agrobacterium-mediated transformation of apple (Malux×domestica Borkh.): an assessment of factors affecting gene transfer efficiency during early transformation steps. Plant Cell Rep., 13: 587–593
Hammerschlag F A, Zimmerman R H, Yadava U L, Hunsucker S, Gercheva P. 1995. An evaluation of antibiotics for the elimination of Agrobacterium tumefaciens from apple leaf explants in vitro and for the effect of regeneration. HortScience, 30: 876
Horsch R B, Fry J E, Hoffmann N L, Eichholtz D, Rogers S G, Fraley R T. 1985. A simple and general method for transferring genes into plants. Science, 227: 1,229–1,231
Ieamkhang S, Chatchawankanphanich O. 2005. Augmentin as an alternative antibiotic for growth suppressionof Agrobacterium for tomato (Lycopersicon esculentum) transformation. Plant Cell Tiss. Organ Cult., 82: 213–220
James D J, Uratsu S, Cheng J, Negri P, Viss P, Dandekar A M. 1993. Acetosyringone and osmoprotectants like betaine or proline synergistically enhance Agrobacterium-mediated transformation of apple. Plant Cell Rep., 12: 559–563
Lin J J, Assad-Garcia N, Kuo J. 1995. Plant hormone effect of antibiotics on the transformation efficiency of plant tissue by Agrobacterium tumefaciens cells. Plant Sci., 109: 171–177
Ling H Q, Kriseleit D, Ganal M W. 1998. Effect of ticarcillin/potassium clavulanate on callus growth and shoot regeneration in Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum Mill.). Plant Cell Rep., 17: 843–847.
Liu G F, Huang J, Chen L Q, Bao M Z. 2002. Plant regeneration from excised hypocotyls explants of Platanus acerifolia Willd. In Vitro Cell Dev. Biol. Plant, 38: 558–563
Liu G, Bao M. 2003. Adventitious shoot regeneration from in vitro cultured leaves of London plane tree (Platanus acerifolia Willd.). Plant Cell Rep., 21: 640–644
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15: 473–497
Nauerbey B, Billing K, Wyndaele R. 1997. Influence of the antibiotic Timentin on plant regeneration compared to carbenicillin and cefotaxime in concentrations suitable for elimination of Agrobacterium tumefaciens. Plant Sci., 123: 169–177
SAS Institute. 1990. SAS user’s guide: statistics. SAS Institute, Cary, NC
Shackelford N J, Chlan CA. 1996. Identification of antibiotics that are effective in eliminating Agrobacterium tumefaciens. Plant Mol. Biol. Rep., 14: 50–57
Shuerman P L, Dandekar A M. 1993. Transformation of temperate woody crops: progress and potentials. Sci Hort., 55: 101–124
Strauss S H, Rottmann W H, Brunner A M, Sheppard L A. 1995. Genetic engineering of reproductive sterility in forest trees. Mol. Breed., 1: 5–26
Sweeney R W, Beech J, Simmons R D. 1988. Pharmacokinetics of intravenously and intramuscularly administered ticarcillin and clavulanic acid in foals. Am. J. Vet. Res., 49: 23–26
Tor M, Mantell H S H, Ainsworth C. 1992. Endophytic bacteria expressing β-glucuronidase cause false positive in transformation of Dioscorea species. Plant Cell Rep., 11: 452–456
Vergauwe A, Cammaert R, Vandenberghe D, Genetello C, Inzé D, Van Montagu M, Van den Eeckhout E. 1996a. Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. and regeneration of transgenic plants. Plant Cell Rep., 15: 929–933
Vergauwe A, Van Geldre E, Inzé D, Van Montagu M, Van den Eeckhout E. 1996b. The use of amoxicillin and ticarcillin in combination with a b-lactamase inhibitor as decontaminating agents in the Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. J. Biotech., 52: 89–95
Zimmerman T W. 1995. Effect of Timentin for controlling Agrobacterium tumefaciens following cocultivation on select plant species. In Vitro Cell Dev. Biol., 31: 70A
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, Zn., Liu, Gf., Fang, F. et al. Adventitious shoot regeneration of Platanus acerifolia Willd. facilitated by Timentin, an antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation. For. Stud. China 9, 14–18 (2007). https://doi.org/10.1007/s11632-007-0003-5
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11632-007-0003-5