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Expression of leptin long-form receptor mRNA in luteinized granulosa cells of obese women with polycystic ovary syndrome

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Summary

To investigate the expression of mRNA of leptin long-form receptor (OB-Rb) in luteinized granulosa cells of obese women with polycystic ovary syndrome (PCOS), and to determine the role of leptin in the physiopathology of PCOS, luteinized granulosa cells were collected from the follicle fluid of 10 obese women who met the diagnostic criteria for PCOS and their BMI was equal to or greater than 25 kg/m2, and at the same time, granulosa cells were collected from 10 normal women undergoing IVF-ET who served as the control group. Some luteinized granulosa cells were taken from normal women for in-vitro culture, into which human leptin of different concentrations was added (0, 10, 100 and 1000 ng/mL). After stimulation with leptin for 48 h, RT-PCR was employed for the detection of the expression of OB-RLmRNA in the luteinized granulosa cells. Our results showed that the level of OB-RLmRNA in luteinized granulosa cells of obese PCOS women was higher than those in the control (P<0.05). In luteinized granulosa cells cultured in vitro and stimulated by human leptin for 48 h, the level of OB-RLmRNA was higher than those without leptin stimulation (P<0.01), and when leptin concentration was at 100 ng/mL, and the level of OB-RLmRNA reached a peak. It is concluded that in obese PCOS women, the level of serum leptin is increased, which promotes the expression of OB-RL in luteinized granulosa cells and increases the sensitivity of the granulosa cells to leptin. Leptin may contribute to anovulation in obese women with PCOS.

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References

  1. Rebecca S B, Susan E M, Paul T et al. Suppression of leptin during lactation: contribution of the suckling stimulus versus milk production. Endocrinology, 1999,140(6): 2621–2627

    Article  Google Scholar 

  2. Friedman J M. Leptin, leptin receptors and the control of body weight. Eur J Med Res, 1997,2(1):7–13

    PubMed  CAS  Google Scholar 

  3. Bűtzow T L, Moilanen J M, Lehtovitrta M et al. Serum and follicular fluid leptin during in vitro fertilization: relationship among leptin increase, body fat mass, and reduced ovarian response. J Clin Endocrinol Metab, 1999,84(9):3135–3139

    Article  PubMed  Google Scholar 

  4. Robert J N, Manny N, Rui** W et al. Improving reproductive performance in overweight/obese women with effective weight management. Hum Reprod Update, 2004,10(3):267–280

    Article  Google Scholar 

  5. Cioffi JA, Van Blerkom J, Antczak M et al. The expression of leptin and its receptor in preovulatory human follicles. Mol Hum Reprod, 1997,3(6):467–472

    Article  PubMed  CAS  Google Scholar 

  6. Hisham A S, Mohamed A, Magdy M et al. Serum leptin elevation in obese women with PCOS: a continuing controversy. J Asist Reprod Genet, 2004,21(10):361–366

    Article  Google Scholar 

  7. Van Blerkom J, Antczak M, Schrader R. The developmental potential of the human oocyte is related to the dissolved oxygen content of follicular fluid: association with vascular endothelial growth factor levels and perifollicular blood flow characteristics. Hum Reprod, 1997,12(5):1047–1055

    Article  PubMed  Google Scholar 

  8. Erel C T, Cline N, Elter K et al. Leptin receptor variant in women with polycystic ovary syndrome. Fertil Steril, 2002,78(6): 1334–1335.

    Article  PubMed  Google Scholar 

  9. Loffler S, Aust G, Kohler U et al. Evidence of leptin expression in normal and polycystic human ovaries. Mol Hum Reprod. 2001,7(12): 1143–1149

    Article  PubMed  CAS  Google Scholar 

  10. Lu X P, Wang B, Huang H F. Expression of leptin mRNA in luteinized granulosa cells and leptin levels in serum and follicular fluid of non-obese infertile patients with polycystic ovary syndrome. Natl Med J China, 2005,85(2):88–91

    CAS  Google Scholar 

  11. Agarwal S K, Vogel K, Weitsman SR et al. Leptin antagonizes the insulin-like growth factor-I augmentation of steroidogenesis in granulosa and theca cells of the human ovary. J Clin Endocrinol Metab, 1999,84(3):1072–1076

    Article  PubMed  CAS  Google Scholar 

  12. Erturk E, Kuru N, Savci V et al. Serum leptin levels correlate with obesity parameters but not with hyperinsulinism in women with polycystic ovary syndrome. Fertil Steril, 2004,82(5):1364–1368

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Yin Jie  (尹婕), Liu Yi  (刘义), Lv Liqun  (吕立群), Wang Donghua  (王冬花), Gong Cheng  (龚成), **ao Wei  (肖维) & Sheng Hui  (盛慧)

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  1. Yin Jie  (尹婕)
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  2. Liu Yi  (刘义)
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  3. Lv Liqun  (吕立群)
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  4. Wang Donghua  (王冬花)
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  5. Gong Cheng  (龚成)
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  6. **ao Wei  (肖维)
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  7. Sheng Hui  (盛慧)
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YIN Jie, female, born in 1981, Master of Medical Sciences

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Yin, J., Liu, Y., Lv, L. et al. Expression of leptin long-form receptor mRNA in luteinized granulosa cells of obese women with polycystic ovary syndrome. J. Huazhong Univ. Sc. Technol. 27, 461–463 (2007). https://doi.org/10.1007/s11596-007-0429-y

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  • DOI: https://doi.org/10.1007/s11596-007-0429-y

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