References
Agarwal, U.P. (2006). Raman imaging to investigate ultrastructure and composition of plant cell walls: distribution of lignin and cellulose in black spruce wood (Picea mariana). Planta 224, 1141–1153.
Chen, F., Tobimatsu, Y., Havkin-Frenkel, D., Dixon, R. A., and Ralph, J. (2012). A polymer of caffeyl alcohol in plant seeds. Proc Natl Acad Sci USA 109, 1772–1777.
Gierlinger, N. (2018). New insights into plant cell walls by vibrational microspectroscopy. Appl Spectr Rev 53, 517–551.
Gierlinger, N., Keplinger, T., and Harrington, M. (2012). Imaging of plant cell walls by confocal Raman microscopy. Nat Protoc 7, 1694–1708.
German, L., Yeshvekar, R., and Benitez-Alfonso, Y. (2023). Callose metabolism and the regulation of cell walls and plasmodesmata during plant mutualistic and pathogenic interactions. Plant Cell Environ 46, 391–404.
**, K., Liu, X., Wang, K., Jiang, Z., Tian, G., Yang, S., Shang, L., and Ma, J. (2018). Imaging the dynamic deposition of cell wall polymer in xylem and phloem in Populus × euramericana. Planta 248, 849–858.
McFarlane, H.E., Doring, A., and Persson, S. (2014). The cell biology of cellulose synthesis. Annu Rev Plant Biol 65, 69–94.
Wang, X., Ren, L., Diao, Z., He, Y., Zhang, J., Liu, M., Li, Y., Sun, L., Chen, R., Ji, Y., et al. (2023). Robust spontaneous Raman flow cytometry for single-cell metabolic phenome profiling via pDEP-DLD-RFC. Adv Sci 10, 2207497.
Wen, X., Chen, Z., Yang, Z., Wang, M., **, S., Wang, G., Zhang, L., Wang, L., Li, J., Saeed, S., et al. (2023). A comprehensive overview of cotton genomics, biotechnology and molecular biological studies. Sci China Life Sci 66, 2214–2256.
Zhao, Y., Man, Y., Wen, J., Guo, Y., and Lin, J. (2019). Advances in imaging plant cell walls. Trends Plant Sci 24, 867–878.
Acknowledgement
This work was supported by the National Natural Science Foundation of China (32030010, 32000558, 32370740), Fundamental Research Funds for the Central Universities (BLX202116 and QNTD202301), Engineering Research & Innovation Team Project of Bei**g Forestry University (NBLRC2023C06), Bei**g Forestry University Outstanding Postgraduate Mentoring Team Building (YJSY-DSTD2022005), Bei**g Municipal Natural Science Foundation (5232016), Bei**g Nova Program (20230484251), Ministry of Science and Technology High-end Foreign Experts Introduction Program (G2022109007L) and the Project Funded by China Postdoctoral Science Foundation (2022T150054, 2023T160054). We thank Prof. Yihua Zhou from the Institute of Genetics and Development Biology, Chinese Academy of Sciences, for generously providing the WT and darx1 of rice plants (Oryza sativa), Ms. Yumei Pu, Dr. Cong Yu and Dr. Chunyang Wang from Horiba Scientific for CRM analysis, Dr. Shoupu Yi and Nengqiang Tang from Zhendian (Suzhou) Medical Technology Co., Ltd for SRS analysis.
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Li, Y., Shen, W., Zhang, X. et al. Single-cell characterization of major components of plant cell walls in situ by Raman spectroscopy. Sci. China Life Sci. (2024). https://doi.org/10.1007/s11427-024-2542-4
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DOI: https://doi.org/10.1007/s11427-024-2542-4