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Stress tolerance and biocontrol performance of the yeast antagonist, Candida diversa, change with morphology transition

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Abstract

As an eco-friendly management method, biological control of postharvest diseases, utilizing antagonistic yeasts, is a research topic receiving considerable attention. Detailed knowledge on the biology of yeast antagonists is crucial when considering their potential application and development as biocontrol products. Changes in the growth form, such as single-cell to pseudohyphae, have been associated with the mode of action in postharvest biocontrol yeasts. In this study, the antagonistic yeast, Candida diversa, reversibly shifted from a single-cell morphology on yeast peptone dextrose (YPD) medium with 2 % agar to a pseudohyphal morphology on YPD with 0.3 % agar. The tolerance of the pseudohyphal form to heat and oxidative stresses, as well as the biocontrol efficacy against Botrytis cinerea on apple and kiwifruit stored at 25 and 4 °C, was significantly higher as compared to the single-cell form. This study provides new information on the ability of C. diversa to change its morphology and the impact of the morphology shift on stress tolerance and biocontrol performance.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 31401794, 31101480 and 31171893), Provincial Natural Science Research Project of Anhui Colleges (No. KJ2014ZD25), a China Postdoctoral Science Foundation Funded Project (No. 2015 M570534), Public Service Platform in Anhui Wanjiang Institute of Poultry Technology (No. 1401032006), and the Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry to Yuan Sui.

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    Authors and Affiliations

    1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, China

      Guangkun Li, Mengshan Chi, Huizhen Chen, Yuan Sui, Yongsheng Liu, Guoqing Liu & Jia Liu

    2. Anhui Wanjiang Institute of Poultry Technology, Hefei University of Technology, Xuancheng Campus, Xuancheng, 242000, China

      Yongsheng Liu, Guoqing Liu & Jia Liu

    3. Yantai Lvyun Biotechnology Co. Ltd, Yantai, 264003, China

      **ao**g Zhang & Zhiqiang Sun

    4. Key Laboratory of Plant Pathology, Ministry of Agriculture, Department of Plant Pathology, China Agricultural University, Bei**g, 100193, China

      Yan Li & Qi Wang

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    1. Guangkun Li
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    Correspondence to Qi Wang or Jia Liu.

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    The authors declare that they have no competing interests.

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    Responsible editor: Philippe Garrigues

    Guangkun Li, Mengshan Chi, Huizhen Chen and Yuan Sui contributed equally to this work.

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    Li, G., Chi, M., Chen, H. et al. Stress tolerance and biocontrol performance of the yeast antagonist, Candida diversa, change with morphology transition. Environ Sci Pollut Res 23, 2962–2967 (2016). https://doi.org/10.1007/s11356-015-5769-8

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    • DOI: https://doi.org/10.1007/s11356-015-5769-8

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