Abstract
Purpose
This study seeks to explore whether chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) can detect liver composition changes between after-meal and over-night-fast statuses.
Procedures
Fifteen healthy volunteers were scanned on a 3.0-T human MRI scanner in the evening 1.5–2 h after dinner and in the morning after over-night (12-h) fasting. Among them, seven volunteers were scanned twice to assess the scan–rescan reproducibility. Images were acquired at offsets (n = 41, increment = 0.25 ppm) from −5 to 5 ppm using a turbo spin echo (TSE) sequence with a continuous rectangular saturation pulse. Amide proton transfer-weighted (APTw) and GlycoCEST signals were quantified with the asymmetric magnetization transfer ratio (MTRasym) at 3.5 ppm and the total MTRasym integrated from 0.5 to 1.5 ppm from the corrected Z-spectrum, respectively. To explore scan time reduction, CEST images were reconstructed using 31 offsets (with 20 % time reduction) and 21 offsets (with 40 % time reduction), respectively.
Results
For reproducibility, GlycoCEST measurements in 41 offsets showed the smallest scan-rescan mean measurements variability, indicated by the lowest mean difference of −0.049 % (95 % limits of agreement, −0.209 to 0.111 %); for APTw, the smallest mean difference was found to be 0.112 % (95 % limits of agreement, −0.698 to 0.921 %) in 41 offsets. Compared with after-meal, both GlycoCEST measurement and APTw measurement under different offset number decreased after 12-h fasting. However, as the offsets number decreased (41 offsets vs. 31 offsets vs. 21 offsets), GlycoCEST map and APTw map became more heterogeneous and noisier.
Conclusion
Our results show that CEST liver imaging at 3.0 T has high sensitivity for fasting.
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Acknowledgments
This study was partially by grants from the Research Grants Council of the Hong Kong SAR (Project No. 476313 and Project No. SEG_CUHK02) and the National Institutes of Health (R01EB009731, R01CA166171).
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Deng, M., Chen, SZ., Yuan, J. et al. Chemical Exchange Saturation Transfer (CEST) MR Technique for Liver Imaging at 3.0 Tesla: an Evaluation of Different Offset Number and an After-Meal and Over-Night-Fast Comparison. Mol Imaging Biol 18, 274–282 (2016). https://doi.org/10.1007/s11307-015-0887-8
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DOI: https://doi.org/10.1007/s11307-015-0887-8