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Evaluation of two fungal exopolysaccharides as potential biomaterials for wound healing applications

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Abstract

Microbial exopolysaccharides (EPSs) are mostly produced by bacteria and fungi and have potential use in the production of biomedical products such as nutraceuticals and in tissue engineering applications. The present study investigated the in vitro biological activities and in vivo wound healing effects of EPSs produced from a Sclerotium-forming fungus (Sclerotium glucanicum DSM 2159) and a yeast (Rhodosporidium babjevae), denoted as scleroglucan (Scl) and EPS-R, respectively. EPS yields of 0.9 ± 0.07 g/L and 1.11 ± 0.4 g/L were obtained from S. glucanicum and R. babjevae, respectively. The physicochemical properties of the EPSs were characterized using infrared spectroscopy and scanning electron microscopy. Further investigations of the biological properties showed that both EPSs were cytocompatible toward the human fibroblast cell line and demonstrated  hemocompatibility. Favorable wound healing capacities of the EPSs (10 mg/mL) were also established via in vivo tests. The present study therefore showed that the EPSs produced by S. glucanicum and R. babjevae have the potential use as biocompatible components for the promotion of dermal wound healing.

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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

M.H would like to acknowledge the postdoctoral fellowship provided by the European Program IF@ULB-MARIE SKŁODOWSKA-CURIE Cofund Action (European Horizon 2020). This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No. 801505. The graphical abstract was prepared using Biorender.com. Also, we must thank Dr. Mahta Mirzaei for her kind help in cell viability assays. The CARAMAT platform at the ULB also received acknowledgement from the authors for its help with SEM investigation.

Funding

This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No. 801505.

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Authors and Affiliations

  1. Université Libre de Bruxelles (ULB), École Polytechnique de Bruxelles, 3BIO-BioMatter, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050, Brussels, Belgium

    Masoud Hamidi, Oseweuba Valentine Okoro & Amin Shavandi

  2. Department of Medical Biotechnology, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran

    Masoud Hamidi & Rasool Mirzaei Seveiri

  3. Research Center of Oils and Fats, Research Institute for Health Technology, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Khodabakhsh Rashidi & Mohammad Saeid Salami

  4. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Hadi Samadian

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  1. Masoud Hamidi
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Contributions

MH: conceptualization, methodology, formal analysis, investigation, validation, writing-original draft, writing-review & editing. OVO: writing-original draft, writing-review & editing. KR: investigation, writing-review & editing. MSS: investigation, writing-review & editing. RMS: investigation, writing-review & editing. HS: investigation, supervision, validation, writing-review & editing. AS: resources, supervision, writing-review & editing, project administration.

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Correspondence to Hadi Samadian or Amin Shavandi.

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The animal experiments were conducted in accordance with the National Institute of Health guidelines and the European Communities Council Directive (2010/63/EU) and approved by Kermanshah University of Medical Sciences (IR.KUMS.REC.1400.247).

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Hamidi, M., Okoro, O.V., Rashidi, K. et al. Evaluation of two fungal exopolysaccharides as potential biomaterials for wound healing applications. World J Microbiol Biotechnol 39, 49 (2023). https://doi.org/10.1007/s11274-022-03459-2

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