Abstract
At present, the study on exopolysaccharid is mainly focused on lactic acid bacteria, and the research on exopolysaccharide produced by yeast, especially Sporidiobolus pararoseus, is relatively few. Therefore, the aim of this study was to explore the characterization and antioxidant activities of a novel neutral exopolysaccharide SPZ, which was isolated and purified from S. pararoseus PFY-Z1. The results showed that SPZ was mainly composed of mannose, followed by glucose, with a molecular weight was 24.98 kDa, had O-glycosidic bonds, no crystalline, and no triple helix structure. Based on fourier transform-infrared, high-performance liquid chromatography and nuclear magnetic resonance analyses, SPZ was identified to be a exopolysaccharide with some side chains, presence of α-, β-pyranose ring and nine sugar residues. Furthermore, the morphology features of SPZ have performed a relatively rough and uneven surface, covered with small pores and fissures. Moreover, SPZ had higher antioxidant activities and the maximum scavenging abilities of ⋅OH, NO2− and reducing power were 28.05 ± 0.73%, 92.76 ± 1.86% and 0.345 ± 0.024, respectively. Hence, SPZ could be used as a potential antioxidant application in the food and pharmaceutical industries.
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Acknowledgements
All appreciation to Qiqihar Medical University, Education Department of Heilongjiang Province, and Qiqihar Science and Technology Bureau. This work was supported by the Fund project of Qiqihar Academy of Medical Sciences (Grant no. [QMSI 2020M-07]), Foundation of Heilongjiang Educational Committee (Grant no. [2021-KYYWF-0381]), and Qiqihar Science and Technology Plan Joint Guidance Project (Grant no. [LSFGG-2022037]).
Funding
This work was supported by the Fund project of Qiqihar Academy of Medical Sciences (Grant No. [QMSI 2020 M-07]), Foundation of Heilongjiang Educational Committee (Grant No. [2021-KYYWF-0381]), and Qiqihar Science and Technology Plan Joint Guidance Project (Grant No. [LSFGG-2022037]).
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Zhenyan Liu, Fangyi Pei, Di Xue, Yuchao Liu, **feng Zhu, Deshui Liu and Hui Li. The first draft of the manuscript was written by Fangyi Pei and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Liu, Z., Pei, F., Zhu, J. et al. Production, characterization and antioxidant activity of exopolysaccharide from Sporidiobolus pararoseus PFY-Z1. World J Microbiol Biotechnol 39, 10 (2023). https://doi.org/10.1007/s11274-022-03453-8
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DOI: https://doi.org/10.1007/s11274-022-03453-8