Abstract
Surface properties like hydrophobicity, aggregation ability, adhesion to mucosal surfaces and epithelial cells and transit time are key features for the characterization of probiotic strains. In this study, we used two Lactobacillus paracasei subsp. paracasei strains (BGNJ1-64 and BGSJ2-8) strains which were previously described with very strong aggregation capacity. The aggregation promoting factor (AggLb) expressed in these strains showed high level of binding to collagen and fibronectin, components of extracellular matrix. The working hypothesis was that strains able to aggregate have an advantage to resist in intestinal tract. So, we assessed whether these strains and their derivatives (without aggLb gene) are able to bind or not to intestinal components and we compared the transit time of each strains in mice. In that purpose parental strains (BGNJ1-64 and BGSJ2-8) and their aggregation negative derivatives (BGNJ1-641 and BGSJ2-83) were marked with double antibiotic resistance in order to be tracked in in vivo experiments in mice. Comparative analysis of binding ability of WT and aggregation negative strains to different human intestinal cell lines and mucin revealed no significant difference among them, excluding involvement of AggLb in interaction with surface of intestinal cells and mucin. In vivo experiments showed that surviving and transit time of marked strains in mice did not drastically depend on the presence of the AggLb aggregation factor.
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Acknowledgements
This work was co-supported by The Ministry of Education, Science and Technological Development of the Republic of Serbia, Republic of Serbia (Grant No. 173019 and Grant for Co-financing of Postdoctoral Training of Researchers in 2017).
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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. Mice experiments were carried out in accordance with the European guidelines for the care and use of laboratory animals (Directive 2010/63/UE). The studies received ethical approval from the local ethics committee (COMETHEA), authorized by the Ministry of Education of Higher Education and Research for the period 2015–2018 (APAFIS # 00680.01). The animal facility was accredited by the Direction des Services Vétérinaires (reference A78-187).
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Miljkovic, M., Thomas, M., Serror, P. et al. Binding activity to intestinal cells and transient colonization in mice of two Lactobacillus paracasei subsp. paracasei strains with high aggregation potential. World J Microbiol Biotechnol 35, 85 (2019). https://doi.org/10.1007/s11274-019-2663-4
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DOI: https://doi.org/10.1007/s11274-019-2663-4