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Differentiation of non-pylori Helicobacter species based on PCR–restriction fragment length polymorphism of the 23S rRNA gene

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Abstract

Phenotypic identification of non-pylori Helicobacter species has always been problematic and time-consuming in comparison with many other bacteria. We developed a rapid two-step identification assay based on PCR–restriction fragment length polymorphism (PCR–RFLP) analysis of the 23S rRNA gene for differentiating between non-pylori Helicobacter species. A new genus-specific primer pair based on all available complete and partial 23S rRNA sequences of Helicobacter species was designed. In silico restriction analysis of variable regions of the 23S rRNA gene suggested SmaI and HindIII endonucleases would provide a good level of differentiation. Analysis of the obtained 23S rRNA RFLP patterns divided all Helicobacter study strains into three species groups (groups A–C) and 12 unique restriction patterns. Wolinella succinogenes also gave a unique pattern. Our proposed PCR–RFLP method was found to be as a valuable tool for routine identification of non-pylori Helicobacter species from human or animal samples.

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Acknowledgments

This work was financially supported by a research grant (Code: 574) from Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

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The authors have no conflict of interest to declare.

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Authors and Affiliations

  1. Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Abbas Yadegar, Masoud Alebouyeh, Tabassom Mirzaei, Ehsan Nazemalhosseini Mojarad & Mohammad Reza Zali

  2. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Abbas Yadegar, Masoud Alebouyeh, Tabassom Mirzaei, Ehsan Nazemalhosseini Mojarad & Mohammad Reza Zali

  3. Gastrointestinal Bacteria Reference Unit, Public Health England, Colindale, London, UK

    Andy J. Lawson

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  1. Abbas Yadegar
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  2. Masoud Alebouyeh
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Correspondence to Masoud Alebouyeh.

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Yadegar, A., Alebouyeh, M., Lawson, A.J. et al. Differentiation of non-pylori Helicobacter species based on PCR–restriction fragment length polymorphism of the 23S rRNA gene. World J Microbiol Biotechnol 30, 1909–1917 (2014). https://doi.org/10.1007/s11274-014-1615-2

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