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Oxygen uptake rate regulation during cell growth phase for improving avermectin B1a batch fermentation on a pilot scale (2 m3)

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Abstract

Avermectin B1a batch fermentation of Streptomyces avermitilis in a 2 m3 fermentor was investigated by oxygen uptake rate (OUR) regulation during cell growth phase. OUR was controlled by adjusting of aeration and agitation. Result showed that OUR strongly affected cell growth and antibiotics production. Avermectin B1a biosynthesis could be effectively enhanced when OUR was stably regulated at an appropriate level in batch fermentation of S. avermitilis. Avermectin B1a yield reached 5568 ± 111 mg/l by controlling maximal OUR between 15 and 20 mmol/l/h during cell growth phase, which was increased by 21.8% compared with the control (maximal OUR above 20 mmol/l/h). The stimulation effect on avermectin B1a production could be attributed to the improved supply of propionic acid and acetic acid, the precursors of avermectin B1a, in the cells. Hence, this OUR control method during cell growth phase may be a simple and applicable way to improve industrial production of avermectin.

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References

  • Burg R, Miller B, Baker E, Birnbaum J, Currie S, Hartman R, Kong Y, Monaghan R, Olson G, Putter I (1979) Avermectins, new family of potent anthelmintic agents: producing organism and fermentation. Antimicrob Agents Chemother 15:361–367

    CAS  Google Scholar 

  • Cane DE, Liang T-C, Kaplan L, Nallin MK, Schulman MD, Hensens O, Douglas AW, Albers-Schoenberg G (1983) Biosynthetic origin of the carbon skeleton and oxygen atoms of the avermectins. J Am Chem Soc 105:4110–4112

    Article  CAS  Google Scholar 

  • Davies RJ, Holdsworth JE, Reader SL (1990) The effect of low oxygen uptake rate on the fatty acid profile of the oleaginous yeast Apiotrichum curvatum. Appl Microbiol Biotechnol 33:569–573

    Article  CAS  Google Scholar 

  • Dumsday GJ, Ocal G, Bridger JS, Zachariou M (2009) The use of oxygen uptake rate to monitor discovery of microbial and enzymatic biocatalysts. Biotechnol Bioeng 102:673–683

    Article  CAS  Google Scholar 

  • Gao H, Liu M, Liu J, Dai H, Zhou X, Liu X, Zhuo Y, Zhang W, Zhang L (2009) Medium optimization for the production of avermectin B1a by Streptomyces avermitilis 14-12A using response surface methodology. Bioresour Technol 100:4012–4016

    Article  CAS  Google Scholar 

  • Higareda AE, Possani LD, Ramirez OT (1997) The use of culture redox potential and oxygen uptake rate for assessing glucose and glutamine depletion in hybridoma cultures. Biotechnol Bioeng 56:555–563

    Article  CAS  Google Scholar 

  • Liang JG, Chu XH, Chu J, Wang YH, Zhuang YP, Zhang SL (2010) Oxygen uptake rate (OUR) control strategy for improving avermectin B1a production during fed-batch fermentation on industrial scale (150 m3). Afr J Biotechnol 9:7186–7191

    CAS  Google Scholar 

  • McKellar QA, Benchaoui HA (1996) Avermectins and milbemycins. J Vet Pharmacol Ther 19:331–351

    Article  CAS  Google Scholar 

  • Novak J, Hajek P, Rezanka T, Vanek Z (1992) Nitrogen regulation of fatty acids and avermectins biosynthesis in Streptomyces avermitilis. FEMS Microbiol Lett 72:57–61

    Article  CAS  Google Scholar 

  • Rajkarnikar A, Kwon HJ, Ryu YW, Suh JW (2007) Two threonine residues required for role of AfsKav in controlling morphogenesis and avermectin production in Streptomyces avermitilis. J Microbiol Biotechnol 17:1563–1567

    CAS  Google Scholar 

  • Schulman MD, Valentino D, Hensens O (1986) Biosynthesis of the avermectins by Streptomyces avermitilis. Incorporation of labeled precursors. J Antibiot (Tokyo) 39:541–549

    CAS  Google Scholar 

  • Takahashi Y, Matsumoto A, Seino A, Ueno J, Iwai Y, Omura S (2002) Streptomyces avermectinius sp nov an avermectin-producing strain. Int J Syst Evol Microbiol 52:2163–2168

    Article  CAS  Google Scholar 

  • **ong Z, Guo M, Guo Y, Chu J, Zhuang Y, Zhang S (2008) Real-time viable-cell mass monitoring in high-cell-density fed-batch glutathione fermentation by Saccharomyces cerevisiae T65 in industrial complex medium. J Biosci Bioeng 105:409–413

    Article  CAS  Google Scholar 

  • **ong Z, Guo M, Guo Y, Chu J, Zhuang Y, Wang N, Zhang S (2010) RQ feedback control for simultaneous improvement of GSH yield and GSH content in Saccharomyces cerevisiae T65. Enzym Microb Technol 46:598–602

    Article  CAS  Google Scholar 

  • Yong JH, Byeon WH (2005) Alternative production of avermectin components in Streptomyces avermitilis by gene replacement. J Microbiol 43:277–284

    CAS  Google Scholar 

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Acknowledgements

This work was financially supported by Major State Basic Research Development Program of China (973 Program, No. 2007CB714303), National Key Technology R&D Program (No. 2007BAI26B02 and No. 2008BAI63B01), National High Technology Research and Development Program of China (863 Program, No. 2009AA032904), and Shanghai Leading Academic Discipline Project (No. B505).

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Jian-Guang Liang, **ao-He Chu, Zhi-Qiang **ong, Ju Chu, Yong-Hong Wang, Ying-** Zhuang & Si-Liang Zhang

  2. School of Biology and Food Engineering, Changshu Institute of Technology, Changshu, 215500, People’s Republic of China

    Jian-Guang Liang

  3. Zhejiang Shenghua Biok Biological Co, Ltd, Huzhou, 313220, People’s Republic of China

    **ao-He Chu

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  1. Jian-Guang Liang
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Correspondence to Ju Chu.

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Liang, JG., Chu, XH., **ong, ZQ. et al. Oxygen uptake rate regulation during cell growth phase for improving avermectin B1a batch fermentation on a pilot scale (2 m3). World J Microbiol Biotechnol 27, 2639–2644 (2011). https://doi.org/10.1007/s11274-011-0737-z

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