Abstract
The series piezoelectric quartz crystal (SPQC) sensing technique is a rapid and sensitive method for detection of microorganisms. In the present study, the detection device was composed of detecting system, signal generating system and data analyzing system. To magnify the amount of detection samples, eight independent SPQC sensors were parallel connected to form a muti-channel detecting unit. Electrodes were separated from the SPQC sensor and immersed into culture medium to detect the change of solution conductivity. The cell constant k was determined as 0.05 m, and the sensitivity interval of the device was from 550 to 600 μs. To maintain sensitivity of the SPQC sensor, a novel culture medium amino acid broth (AaB) was developed. It was nutrient with low conductivity and satisfied our detection device. For determining frequency detection time (FDT) expediently and accurately, FDT was defined afresh with fitting–differentiating method. Pathogens Staphylococcus aureus and Shigella dysenteriae were determined with an automated detecting device and the methods mentioned above. The calibration curves of FDT against density of bacteria showed a linear correlation coefficient (R ≥ 0.99) over the range of 10–106 cells ml−1. Detection results all fell inside the 95% confidence interval of a standard pour plate counting method. The reproducibility was also reviewed, and results showed that the device was stable and sensitive even after 180 days of employment.
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References
Bao L, Deng L, Nie L, Yao S, Wei W (1996) Determination of microorganisms with a quartz crystal microbalance sensor. Anal Chim Acta 319:97–101
Bao L, Tan H, Duan Q, Su X, Wei W (1998a) A rapid method for determination of Staphylococcus aureus based on milk coagulation by using a series piezoelectric quartz crystal sensor. Anal Chim Acta 374:47–52
Bao L, Tan H, Deng L, Wei W (1998b) A new piezoelectric response model for population growth of bacteria. Talanta 47:267–273
Chang K, Jang H, Lee C, Lee Y, Yuan C, Lee S (2006) Series quartz crystal sensor for remote bacteria population monitoring in raw milk via the Internet. Biosens Bioelectron 21:1581–1590
Chen P, Nie L, Yao S (1995a) Application of a series piezoelectric sensor as a detector in ion chromatography. J Chromatogr Sci 33:268–272
Chen P, Nie L, Yao S (1995b) Determination of lactic acid and pyruvic acid in serum and cerebrospinal fluid by ion-exclusion chromatography with a bulk acoustic wave detector. J Chromatogr B Biomed Appl 673:153–158
Deng L, He F, Jiang T, Nie L, Yao S (1995) A goat-anti-human IgG modified piezoelectric immunosensor for Staphylococcus aueus detection. J Microbiol Methods 23:229–234
Duman M, Saber R, Piskin E (2003) A new approach for immobilization of oligonucleotides onto piezoelectric quartz crystal for preparation of a nucleic acid sensor for following hybridization. Biosens Bioelectron 18:1355–1363
Faruque SM, Chowdhury N, Khan R, Hasan MR, Nahar J, Islam MJ, Yamasaki S, Ghosh AN, Nair GB, Sack DA (2003) Shigella dysenteriae type 1-specific bacteriophage from environmental waters in bangladesh. Appl Environ Microbiol 69:7028–7031
He F, Geng Q, Zhu W, Nie L, Yao S, Chang M (1994) Rapid detection of Escherichia coli using a separated electrode piezoelectric crystal sensor. Anal Chim Acta 289:313–319
He F, Deng L, Nie L, Yao S (1995) Rapid detection of Staphylococcus aureus using a separated electrode piezoelectric crystal sensor. Anal Lett 28:213–224
He F, Zhao J, Zhang L, Su X (2003) A rapid method for detection Mycobacterium tuberculosis based on a bulk acoustic wave impendence biosensor. Talanta 59:935–941
Horan T, Culver D, Jarvis W (1988) Pathogens causing nosocomial infections: preliminary data from the national nosocomial infections surveillance system. Antimicrobiol Newsl 5:65–67
King WH (1964) Piezoelectric sorption detector. Anal Chem 36:1735–1739
Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, Griffin PM, Tauxe RV (1999) Food-related illness and deaths in the United States. Emerg Infect Dis 5:605–625
Mo X, Zhou Y, Lei H, Deng L (2002) Microbalance-DNA probe method for the detection of specific bacteria in water. Enzyme Microb Technol 30:583–589
Qu X, Bao L, Su X, Wei W (1998) A new method based on gelation of tachypleus amebocyte lysate for detection of Escherichia coli form using a series piezoelectric quartz crystal sensor. Anal Chim Acta 374:47–52
Sauerbrye G (1959) The use of quartz oscillators for weighing thin layers and for microweighing. Z Phys Chem 155:206–222
Shen DZ, Zhu WH, Nie LH, Yao S (1993) Behaviour of a series piezoelectric sensor in electrolyte solution. Anal Chim Acta 276:87–97
Wu Y, Yi L, **e QJ, Zhang Y, Yin F, Yao S (2001) Monitoring of DNA oxidative damage with piezoelectric quartz crystal method. Talanta 54:263–270
Xu Y, Lu C, Chen K, Nie L, Yao S (1996) A conductance sensor for dissolved sulphur dioxide using a series piezoelectric crystal device. Talanta 43:1297–1303
Yao SZ, Nie L (1987) Oscillation behavior of a piezoelectric crystal in liquids and its applications. Anal Proc 24:336–337
Yao SZ, Zhou TA (1988) Dependence of the oscillation frequence of a piezoelectric crystal on the physical properties of liquids. Anal Chem Acta 212:61–67
Zhang J, Bao L, Wei W (1999) A series piezoelectric quartz crystal microbial sensing technique used for biochemical oxygen demand assay in environmental monitoring. Microchem J 62:405–412
Zhang JZ, Wei WZ, Zhou A, He D, Yao S, **e Q (2000) Monitoring of mutagenic process with piezoelectric quartz crystal impedance analysis. Talanta 53:525–533
Zhang J, **e Y, Dai X, Wei W (2001) Monitoring of Lactobacillus fermentation process by using ion chromatography with a series piezoelectric quartz crystal detector. J Microbiol Methods 44:105–111
Zhang S, Wei W, Zhang J, Mao Y, Liu S (2002) Effect of static magnetic field on growth of Escherichia coli and relative response model of series piezoelectric quartz crystal. Analyst 127:373–377
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This research was funded by the National Natural Science Foundation of China (No. 20477059) and the Doctoral Fellow Foundation, State Education Ministry of China (20040533048).
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Zhu, W., Chen, L., Yang, Z. et al. Automatic detection of Staphylococcus aureus and Shigella dysenteriae with separated electrodes series piezoelectric sensing technique. World J Microbiol Biotechnol 24, 1073–1079 (2008). https://doi.org/10.1007/s11274-007-9608-z
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DOI: https://doi.org/10.1007/s11274-007-9608-z