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Complete genome and molecular characterization of a putative novel citrivirus from Rudbeckia sp.

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Abstract

We identified a tentative novel positive-strand RNA virus from Rudbeckia sp., namely, Rudbeckia citrivirus A (RuCVA). The complete genome sequence of the novel virus was 8821 nucleotides in length, excluding the poly(A) tail. It has three open reading frames (ORFs): a putative polyprotein, a movement protein, and a coat protein. Phylogenetic analysis showed that the virus was more closely related to Citrus leaf blotch virus isolates and unassigned citriviruses. The sequence identity of the virus with other citriviruses was lower than 56.9% at the complete nucleotide sequence level. For each ORF, the sequence identity was lower than 64.2% at the nucleotide level and 67.8% at the amino acid level. These results satisfied the species demarcation criteria for Betaflexiviridae. Therefore, we suggest that RuCVA is a novel member of the genus Citrivirus.

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Acknowledgements

We would like to thank Editage (www.editage.co.kr) for the English language editing.

Funding

This work was supported by a research fund (project no. B-1543086–2021-22–01) from the Animal and Plant Quarantine Agency, Ministry of Agriculture, Food, and Rural Affairs, Republic of Korea.

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Authors and Affiliations

  1. Plant Quarantine Technology Center, Department of Plant Quarantine, Animal and Plant Quarantine Agency, Gimcheon, 39660, Republic of Korea

    Juhyun Kim, Minji Jun, Da-Som Lee, Eun ** Jeon, Sanghyun Shin, Seong-** Lee & Seungmo Lim

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  1. Juhyun Kim
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  2. Minji Jun
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Contributions

SL and S-JL contributed to the study conception and design. JK, MJ, D-SL, EJJ, SS, and SL performed the experiments, data collection, and analysis. JK and SL wrote the manuscript. All the authors have read and approved the final manuscript.

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Correspondence to Seungmo Lim.

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The authors have no relevant financial or non-financial interests to disclose.

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This article does not describe any studies involving human participants or animals performed by any of the authors.

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Edited by Seung-Kook Choi.

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Kim, J., Jun, M., Lee, DS. et al. Complete genome and molecular characterization of a putative novel citrivirus from Rudbeckia sp.. Virus Genes 59, 158–162 (2023). https://doi.org/10.1007/s11262-022-01936-2

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