Abstract
Cotesia vestalis (Haliday) (Hymenoptera: Braconidae) is an endoparasitoid of the larval stage of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae) and injects a polydnavirus (CvBV) into its host during oviposition. In this paper we characterize CvBV202 and its product. CvBV202 is located on segment S2 of CvBV genome; it has a size of 984 bp and encodes a putative protein of 328 amino acids, including protein phosphatase domain and tyrosine-specific protein phosphatase domain. Gene transcripts were detected in extracts of the host as early as 2 h post-parasitization (p.p.) and continued to be detected for 6 days. Tissue-specific patterns of this gene expression showed that CvBV202 had a close relationship with the host’s physiological alternations including immunosuppression, modulation of hormone titer, and nutrition metabolism. The protein was detected in the parasitized hosts at 12 h p.p. using western blot assay. The product of CvBV202 was found to be around 59 kDa, much larger than the predicted molecular weight of 37.8 kDa, suggesting that post-translational modification of CvBV202 occurs in host cells, corresponding with the existence of many post-translational modification sites. Immunofluorescence staining and confocal laser scanning microscopy revealed that CvBV202 and the fused protein eGFP-CvBV202 were observed both in the nuclear region and cytoplasm of the hemocytes of the naturally parasitized host larvae and rBac-eGFP-CvBV202-infected Tn-5B1-4 cells, respectively.
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Acknowledgment
We are grateful to Dr. M. Sharkey (University of Kentucky) for his critical reading of an early draft of the manuscript, Ms Zaidi Farrah for improving the English text, and Professor C.X. Zhang and Dr Z.N. Yang (Institute of Insect Science, Zhejiang University) for their help in the experimental stage of this research. We also thank three anonymous referees for their invaluable comments. Funding for this study was provided jointly by 973 Program (2006CB102005), Key Program of Natural Science Foundation of Zhejiang Province (Z306031), National Science Funds for Distinguished Young Scholars (Grant No.30625006), Program for New Century Excellent Talents in University (NCET-04-0521), Innovation Research Team Program of the Ministry of Education of China (IRT0355), and the China Postdoctoral Science Foundation (20060400322).
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Shi, M., Chen, YF., Yao, Y. et al. Characterization of a protein tyrosine phosphatase gene CvBV202 from Cotesia vestalis polydnavirus (CvBV). Virus Genes 36, 595–601 (2008). https://doi.org/10.1007/s11262-008-0225-1
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DOI: https://doi.org/10.1007/s11262-008-0225-1