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Somatic embryogenesis and plant regeneration from suspension cultures of Sapindus trifoliatus

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Abstract

A procedure for plant regeneration from cell suspension cultures through somatic embryogenesis is described for Sapindus trifoliatus, a commercially and medicinally important tree. Callus was induced from leaf disc on agar-solidified MS medium with 5.0 mg l−1 2, 4-D and 0.01 mg l−1 Kin. Embryogenic cell suspension cultures were established by placing leaf-derived friable calli in PGR-free full-strength MS liquid medium with 3% sucrose. The growth of cell suspension culture was significantly affected by the strength of the MS mineral solution and L-glutamine. Plating of the suspension on semisolid MS medium resulted in the formation of globular structures. These embryogenic globular structures differentiated into secondary globular structures or somatic embryos on a semisolid MS medium. The differentiation of globular structures and different stages of somatic embryos (from globular to cotyledonary) was enhanced by the addition of 200 mg l−1 L-glutamine in the medium. Sucrose at relatively high concentrations (5%) or ABA (0.01 mg l−1) promoted somatic embryo maturation. The highest percentage (about 90%) of germination of somatic embryo and plantlet conversion was achieved on a half-strength MS medium containing 2% sucrose. The plants were hardened and established in soil with a 90% survival rate

Key message

The present study demonstrates the establishment of suspension culture from leaf-derived callus in Sapindus trifoliatus and plant regeneration via somatic embryogenesis

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Funding

This work was supported by the Council of Scientific & Industrial Research (CSIR), India.

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Author notes

  1. Madhulika Singh & Pooja Asthana contributed equally to this work and should be considered as co-first authors.

Authors and Affiliations

  1. Laboratory of Morphogenesis, Department of Botany, Banaras Hindu University, Varanasi, 221 005, Uttar Pradesh, India

    Madhulika Singh & Pooja Asthana

  2. Department of Environmental Science, Indira Gandhi National Tribal University, Amarkantak, 484887, M.P, India

    Manoj K. Rai

  3. Department of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India

    Uma Jaiswal

  4. Department of Life Sciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur, UP, 208 024, India

    Madhulika Singh

  5. Department of Botany, Ramsheth Thakur College of Commerce and Science, Kharghar, Navi Mumbai, 410210, Maharashtra, India

    Pooja Asthana

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  1. Madhulika Singh
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Contributions

Conceptualization: MS, PA, UJ; Methodology & Experimentation: MS, PA; Formal analysis: MS, PA, MKR; Writing–original draft: MKR, PA; Writing–review and editing: MS; Supervision: UJ.

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Correspondence to Madhulika Singh or Manoj K. Rai.

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Communicated by :Klaus Eimert.

The work is dedicated to the late Prof. V.S. Jaiswal (1945–2007).

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Singh, M., Asthana, P., Rai, M.K. et al. Somatic embryogenesis and plant regeneration from suspension cultures of Sapindus trifoliatus. Plant Cell Tiss Organ Cult 157, 36 (2024). https://doi.org/10.1007/s11240-024-02760-2

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