Abstract
Obtaining citrus polyploids, especially from triploid individuals, is of great importance for genetic improvement programs, as they produce seedless fruits. Therefore, the aim of this study was to use flow cytometry analyses to identify polyploid individuals among tangerine-type seedlings originating from seeds germinated in vitro. Fruits were collected from 13 different genotypes in the municipality of Mucugê (state of Bahia, Brazil). The seeds were then removed and classified as developed (seeds with well-formed integument), underdeveloped (seeds with wrinkled integument) and small (seeds of 1/3 to 1/6 of normal size). After the integument was removed, the seeds were disinfected and introduced into a WPM culture medium, in which they remained for 120 days. After this period, the percentage of germinated seeds and growth variables were evaluated, and ploidy analysis was conducted using flow cytometry. The developed seeds displayed a higher germination rate (91.5%) than the others. The small and underdeveloped seeds resulted in a considerable frequency of spontaneous polyploids (11%), and one haploid plant was also obtained from the latter class.
Key message
The ploidy of 13 citrus genotypes was investigated using flow cytometry. In addition to polyploid plants that are of interest to breeding programs, there was the identification of a haploid plant.
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Data availability
All data generated were analyzed during this study and are included in this published article.
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Funding
To CAPES and FAPESB, for their financial support for the scholarships of the students involved in the study. To Embrapa Mandioca e Fruticultura, for providing the physical structure and financial support for the development of the study. To UESC (Universidade Estadual de Santa Cruz), specifically to the Graduate Program in Crop Production, for supporting the development of the thesis from which this article was extracted.
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DSVV, MISM and FFA collected the data and interpreted the results. DSVV and MISM wrote the manuscript. DSVV, MISM, ASS, KCFS and FFA provided technical support to set up and conduct the study. ASS, KCFS, ASG, WSSF and MGCC defined the main lines of the experiments. MISM supported the statistical analysis. MGCC, ASS and KCFS were the supervisors of this research. MGCC, ASS, WSSF, ASG and KCFS contributed with the financial acquisition of the experiments.
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Communicated by Maria Antonietta Germanà.
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dos Santos Vila Verde, D., de Souza Mendes, M.I., da Silva Souza, A. et al. Obtaining spontaneous haploids and polyploids from seeds derived from tangerine-type fruits. Plant Cell Tiss Organ Cult 153, 133–144 (2023). https://doi.org/10.1007/s11240-023-02449-y
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DOI: https://doi.org/10.1007/s11240-023-02449-y