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Expression Analysis of Two NAC Transcription Factors PtNAC068 and PtNAC154 from Poplar

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Abstract

NAM/ATAF/CUC (NAC) family genes comprise one of the largest families of transcription factors in plant genomes and are widely expressed in develo** woody tissues. In the present study, we constructed plant transformation vectors using the β-glucuronidase (GUS) reporter gene system and detected the promoter expression patterns derived from the PtNAC068 and PtNAC154 genes of Chinese white poplar (Populus tomentosa Carr.) in transgenic poplars (Populus alba × Populus glandulosa). The results showed that the GUS expression driven by PtNAC068 and PtNAC154 promoters may be more complex in poplar than they are in Arabidopsis. Histochemical GUS assays showed that GUS activity driven by PtNAC068 promoter was mainly in vascular tissues of stems, leaves, petioles, and roots, while that driven by PtNAC154 promoter was confined to the develo** secondary xylem of stems and veins of leaves. The transcript level of both PtNAC068 and PtNAC154 in successive internodes below the apex was found to be much higher in IN5-10 compared to that in IN2-4 as measured by real-time RT-PCR, suggesting that PtNAC068 and PtNAC154 upregulation is related to secondary growth in poplar. GUS expression in internodes 3–8 of ProNAC068::GUS transgenic plants was 30-fold higher than that in ProNAC154::GUS transgenic plants. The differences in the expression pattern and transcript level of mRNA accumulation indicate that PtNAC068 and PtNAC154 may be involved in two distinct aspects of vascular tissue development.

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Abbreviations

aa:

Amino acid

CTAB:

Cetyl trimethylammonium bromide

GUS:

β-Glucuronidase

IN:

Internode

NAC:

NAM/ATAF/CUC

NPTII:

Neomycin phosphotransferase II

RT-qPCR:

Real-time quantitative PCR

X-gluc:

5-Bromo-4-chloro-3-indoxyl-beta-d-glucuronic acid

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Acknowledgement

The work presented here was supported by the Natural National Science Foundation of China (31030018).

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Authors and Affiliations

  1. International Centre for Bamboo and Rattan, Bei**g, 100102, People’s Republic of China

    **aojiao Han & Changhua Guo

  2. State Key Laboratory of Forest Genetics and Tree Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Bei**g, 100091, People’s Republic of China

    **aojiao Han, Guo He, Shutang Zhao, Changhua Guo & Mengzhu Lu

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  1. **aojiao Han
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  2. Guo He
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  3. Shutang Zhao
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Correspondence to Mengzhu Lu.

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Han, X., He, G., Zhao, S. et al. Expression Analysis of Two NAC Transcription Factors PtNAC068 and PtNAC154 from Poplar. Plant Mol Biol Rep 30, 370–378 (2012). https://doi.org/10.1007/s11105-011-0350-1

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