Abstract
Purpose
This study was conducted to understand the effects of water and nitrogen (N) on net root productivity, root longevity, root length, and canopy characteristics of cotton (Gossypium hirsutum L.), and to quantify the associations between canopy characteristics and indicators of root dynamics.
Methods
A two-year field experiment was performed to evaluate the variation in canopy growth and root dynamics of cotton in response to three nitrogen levels (320 kg ha−1, high N; 272 kg ha−1, moderate N; 224 kg ha−1, low N) and two water levels (400 mm, high-water group; 267 mm, low-water group) during the growing seasons of 2019 and 2020 in **njiang, China.
Results
Plants exposed to low-water environments increased root longevity more than all other high-water treatments. Under high-water conditions, a moderate N rate obtained a higher root longevity compared to high-N and low-N treatments. Net root productivity (NRP) was strongly associated with leaf area expansion rate and leaf number. A moderate N rate under high-water conditions exhibited a significant 30.0% increase in NRP, resulting in a relatively high leaf area and the highest leaf relative water content, and had an equivalent canopy photosynthetic rate compared with high-N plots. Plants receiving moderate N in the high-water group had greater total root length by increasing root longevity and productivity.
Conclusions
Estimates of NRP may need to be included along with leaf growth estimates in future efforts to scale from root to canopy level developmental performance in cotton.
Graphical abstract
Similar content being viewed by others
Data availability
The complete data file is held by the first author, who may be reached at the following email address: baojian92@qq.com.
Code availability
Not applicable.
References
Anderson LJ, Comas LH, Lakso AN, Eissenstat DM (2003) Multiple risk factors in root survivorship: a 4-year study in Concord grape. New Phytol 158:489–501. https://doi.org/10.1046/j.1469-8137.2003.00757.x
Ansley RJ, Boutton TW, Jacoby PW (2014) Root biomass and distribution patterns in a semi-arid mesquite savanna: responses to long-term rainfall manipulation. Rangeland Ecol Manag 67:206–218. https://doi.org/10.2111/REM-D-13-00119.1
Angadi SV, Umesh MR, Begna S, Gowda P (2022) Light interception, agronomic performance, and nutritive quality of annual forage legumes as affected by shade. Field Crops Res 275:108358. https://doi.org/10.1016/j.fcr.2021.108358
Baddeley JA, Watson CA (2005) Influences of root diameter, tree age, soil depth and season on fine root survivorship in Prunus avium. Plant Soil 276:15–22. https://doi.org/10.1007/s11104-005-0263-6
Bondada BR, Oosterhuis DM, Norman RJ, Baker WH (1996) Canopy photosynthesis, growth, yield, and boll 15N accumulation under nitrogen stress in cotton. Crop Sci 36(1):127–133. https://doi.org/10.2135/cropsci1996.0011183X003600010023x
Burton AJ, Pregitzer KS, Hendrick RL (2000) Relationships between fine root dynamics and nitrogen availability in Michigan northern hardwood forests. Oecologia 125:389–399. https://doi.org/10.1007/s004420000455
Chen W, Chen F, Lai S, ** M, Xu S, Liu Y, Liang X, Ferré TP (2022) Spatial distribution and dynamics of cotton fine root under film-mulched drip irrigation. Ind Crop Prod 179:114693. https://doi.org/10.1016/j.indcrop.2022.114693
Carrillo Y, Dijkstra FA, LeCain D, Morgan JA, Blumenthal D, Waldron S, Pendall E (2014) Disentangling root responses to climate change in a semiarid grassland. Oecologia 175:699–711. https://doi.org/10.1007/s00442-014-2912-z
Chen HYH, Brassard BW (2013) Intrinsic and extrinsic controls of fine root life span. Crit Rev Plant Sci 32:151–161. https://doi.org/10.1080/07352689.2012.734742
Chen G, Peng Y, Zheng J, Li S, Peng T, Qiu X, Tu L (2017) Effects of short-term nitrogen addition on fine root biomass, lifespan and morphology of Castanopsis platyacantha in a subtropical secondary evergreen broad-leaved forest. Chin J Plant Ecol 41:1041–1050. https://doi.org/10.17521/cjpe.2016.0317 (in chinese)
Chen J, Liu L, Wang Z, Zhang Y, Sun H, Song S, Bai Z, Lu Z, Li C (2020) Nitrogen fertilization increases root growth and coordinates the root–shoot relationship in cotton. Front Plant Sci 11:880. https://doi.org/10.3389/fpls.2020.00880
Chen J, Liu L, Wang Z, Sun H, Zhang Y, Lu Z, Li C (2018) Determining the effects of nitrogen rate on cotton root growth and distribution with soil cores and minirhizotrons. PLoS One 13(5):e0197284. https://doi.org/10.1371/journal.pone.0197284
Chen G, Rasmussen CR, Dresboell DB, Smith AG, Thorup-Kristensen K (2021a) Dynamics of deep water and N uptake under varied N and water supply. bioRxiv. https://doi.org/10.1101/2021.09.27.461951
Chen G, Dresbøll DB, Thorup-Kristensen K (2021b) Dual labelling by 2H and 15N revealed differences in uptake potential by deep roots of chicory. Rhizosphere 19:100368. https://doi.org/10.1016/j.rhisph.2021.100368
Craine JM, Dybzinski R (2013) Mechanisms of plant competition for nutrients, water and light. Funct Ecol 27:833–840. https://doi.org/10.1111/1365-2435.12081
Clément C, Sleiderink J, Svane SF, Smith AG, Diamantopoulos E, Desbrøll DB, Thorup-Kristensen K (2022) Comparing the deep root growth and water uptake of intermediate wheatgrass (Kernza®) to alfalfa. Plant Soil 479:1–22. https://doi.org/10.1007/s11104-021-05248-6
Cui Z, Zhang H, Chen X et al (2018) Pursuing sustainable productivity with millions of smallholder farmers. Nature 555(7696):363–366. https://doi.org/10.1038/nature25785
Crawley MJ (2012) The R Book, Second Edition. 27. Survival analysis, Wiley, Hoboken , p 1218
Dresbøll DB, Thorup-Kristensen K, McKenzie BM, Dupuy LX, Bengough AG (2013) Timelapse scanning reveals spatial variation in tomato (Solanum lycopersicum L.) root elongation rates during partial waterlogging. Plant Soil 369(1):467–477. https://doi.org/10.1007/s11104-013-1592-5
Dresbøll DB, Rasmussen IS, Thorup-Kristensen K (2016) The significance of litter loss and root growth on nitrogen efficiency in normal and semi-dwarf winter oilseed rape genotypes. Field Crop Res 186:166–178. https://doi.org/10.1007/10.1016/j.fcr.2015.12.003
Freschet GT, Roumet C, Comas LH et al (2021) Root traits as drivers of plant and ecosystem functioning: Current understanding, pitfalls and future research needs. New Phytol 232:1123–1158. https://doi.org/10.1111/nph.17072
Gaul D, Hertel D, Borken W, Matzner E, Leuschner C (2008) Effects of experimental drought on the fine root system of mature Norway spruce. Forest Ecol Manag 256(5):1151–1159. https://doi.org/10.1016/j.foreco.2008.06.016
Graefe S, Hertel D, Leuschner C (2008) Estimating fine root turnover in tropical forests along an elevational transect using minirhizotrons. Biotropica 40(536):542. https://doi.org/10.1111/j.1744-7429.2008.00419.x
Goel MK, Khanna P, Kishore J (2010) Understanding survival analysis: Kaplan-Meier estimate. Int J Ayurveda Res 1:274–278. https://doi.org/10.4103/0974-7788.76794
Germon A, Cardinael R, Prieto I, Prieto I, Mao Z, Kim J, Stokes A, Dupraz C, Laclau J, Jourdan C (2016) Unexpected phenology and lifespan of shallow and deep fine roots of walnut trees grown in a silvoarable Mediterranean agroforestry system. Plant Soil 401:409–426. https://doi.org/10.1007/s11104-015-2753-5
Houde S, Thivierge MN, Fort F, Bélanger G, Chantigny MH, Angers DA, Vanasse A (2020) Root growth and turnover in perennial forages as affected by management systems and soil depth. Plant Soil 451(1):371–387. https://doi.org/10.1007/s11104-020-04532-1
Hendrick RL, Pregitzer KS (1993) Patterns of fine root mortality in two sugar maple forests. Nature 361:59–61. https://doi.org/10.1038/361059a0
Hoffmann CM (2014) Adaptive responses of Beta vulgaris L. and Cichorium intybus L. root and leaf forms to drought stress. J Agron Crop Sci 200(2):108–118. https://doi.org/10.1111/jac.12051
Huot C, Zhou Y, Philp JN, Denton MD (2020) Root depth development in tropical perennial forage grasses is related to root angle, root diameter and leaf area. Plant Soil 456(1):145–158. https://doi.org/10.1007/s11104-020-04701-2
Heeraman DA, Juma NG (1993) A comparison of minirhizotron, core and monolith methods for quantifying barley (hordeum vulgare l.) and fababean (vicia faba l.) root distribution. Plant Soil 148(1):29–41. https://doi.org/10.1007/BF02185382
Hulugalle NR, Broughton KJ, Tan DK (2015) Fine root production and mortality in irrigated cotton, maize and sorghum sown in vertisols of northern New South Wales, Australia. Soil till Res 146:313–322. https://doi.org/10.1016/j.still.2014.10.004
Jørgensen L, Dresbøll DB, Thorup-Kristensen K (2014) Spatial root distribution of plants growing in vertical media for use in living walls. Plant Soil 380(1):231–248. https://doi.org/10.1007/s11104-014-2080-2
Johnson MG, Tingey DT, Phillips DL, Storm MJ (2001) Advancing fine root research with minirhizotrons. Environ Exp Bot 45:263–289. https://doi.org/10.1016/S0098-8472(01)00077-6
Keel SG, Campbell CD, Högberg MN, Richter A, Wild B, Zhou X, Hurry V, Linder S, Näsholm T, Högberg P (2012) Allocation of carbon to fine root compounds and their residence times in a boreal forest depend on root size class and season. New Phytol 194:972–981. https://doi.org/10.1111/j.1469-8137.2012.04120.x
Lambers H, Atkin OK, Millenaar FF (2002) Respiratory patterns in roots in relation tot heir functioning. In: Waisel Y, Eshel A, Kafkaki K (eds) Plant Roots, Hidden Half, Third Edit. Marcel Dekker Inc, New York, pp 521–552. https://doi.org/10.1201/9780203909423.pt6
Li WB, ** CJ, Guan DX, Wang QK, Wang AZ, Yuan FH, Wu JB (2015) The effects of simulated nitrogen deposition on plant root traits: a meta-analysis. Soil Biol Biochem 82:112–118. https://doi.org/10.1016/j.soilbio.2015.01.001
Lobell DB, Roberts MJ, Schlenker W, Braun N, Little BB, Rejesus RM, Hammer GL (2014) Greater sensitivity to drought accompanies maize yield increase in the U.S. Midwest. Science 344:516–519. https://doi.org/10.1126/science.1251423
Liu H, Lin L, Wang H, Zhang Z, Shangguan Z, Feng X, He JS (2021) Simulating warmer and drier climate increases root production but decreases root decomposition in an alpine grassland on the Tibetan plateau. Plant Soil 458(1):59–73. https://doi.org/10.1007/s11104-020-04551-y
Lu B, Qian J, Hu J, Wang P, ** W, Tang S, He Y, Zhang C (2022) The role of fine root morphology in nitrogen uptake by riparian plants. Plant Soil 472:1–16. https://doi.org/10.1007/s11104-021-05270-8
Lukac M (2012) Fine root turnover. Measuring roots. Springer, Berlin, Heidelberg, pp 363–373
Luo Y, Wang X, Cui M, Wang J, Gao Y (2021) Mowing increases fine root production and root turnover in an artificially restored Songnen grassland. Plant Soil 465(1):549–561. https://doi.org/10.1007/s11104-021-05017-5
Lynch JP (2007) Rhizoeconomics: the roots of shoot growth limitations. HortScience 42:1107–1109
McCormack ML, Dickie IA, Eissensat DM et al (2015) Redefining fine roots improves understanding of belowground contributions to terrestrial biosphere processes. New Phytol 207:505–518. https://doi.org/10.1111/nph.13363
McCormack ML, Guo D (2014) Impacts of environmental factors on fine root lifespan. Front Plant Sci 5:205. https://doi.org/10.3389/fpls.2014.00205
McCormack ML, Guo D, Iversen CM et al (2017) Building a better foundation: improving root-trait measurements to understand and model plant and ecosystem processes. New Phytol 215:27–37. https://doi.org/10.1111/nph.14459
Mueller KE, LeCain DR, McCormack ML, Pendall E, Carlson M, Blumenthal DM (2018) Root responses to elevated CO2, warming and irrigation in a semi-arid grassland: Integrating biomass, length and life span in a 5-year field experiment. J Ecol 106(6):2176–2189. https://doi.org/10.1111/1365-2745.12993
Merrill SD, Tanaka DL, Hanson JD (2002) Root length growth of eight crop species in Haplustoll soils. Soil Sci Soc Am J 66(3):913–923. https://doi.org/10.2136/sssaj2002.0913
Nadelhoffer KJ (2000) The potential effects of nitrogen deposition on fine-root production in forest ecosystems. New Phytol 147:131–139. https://doi.org/10.1046/j.1469-8137.2000.00677.x
Nelson DW, Sommers LE (1973) Determination of total nitrogen in plant material. Agronomy J 65:109–112. https://doi.org/10.2134/agronj1973.00021962006500010033x
Oldroyd GED, Leyser O (2020) A plant`s diet, surviving in a variable nutrient environment. Science 368:6486. https://doi.org/10.1126/science.aba0196
Padilla FM, Pugnaire FI (2007) Rooting depth and soil moisture control Mediterranean woody seedling survival during drought. Funct Ecol 21:489–495. https://doi.org/10.1111/j.1365-2435.2007.01267.x
Pinto P, Rubio G, Gutiérrez F, Sawchik J, Arana S, Piñeiro G (2021) Variable root: shoot ratios and plant nitrogen concentrations discourage using just aboveground biomass to select legume service crops. Plant Soil 463(1):347–358. https://doi.org/10.1007/s11104-021-04916-x
Pregitzer KS, Laskowski MJ, Butron AJ, Lessard VC, Zak DR (1998) Variation in sugar maple root respiration with root diameter and soil depth. Tree Physiol 18:665–670. https://doi.org/10.1093/treephys/18.10.665
Plett DC, Ranathunge K, Melino VJ, Noriyuki K, Yusaku U, Kronzucker HJ (2020) The intersection of nitrogen nutrition and water use in plants: new paths toward improved crop productivity. J Exp Bot 71:4452–4468. https://doi.org/10.1093/jxb/eraa049
Roumet C, Birouste M, Picon CC, Ghestem M, Osman N, Vrignon BS, Cao K, Stokes A (2016) Root structure–function relationships in 74 species: evidence of a root economics spectrum related to carbon economy. New Phytol 210:815–826. https://doi.org/10.1111/nph.13828
Rasmussen CR, Thorup-Kristensen K, Dresbøll DB (2020) Uptake of subsoil water below 2 m fails to alleviate drought response in deep-rooted Chicory (Cichorium intybus L.). Plant Soil 446(1):275–290. https://doi.org/10.1007/s11104-019-04349-7
Rochester IJ (2011) Assessing internal crop nitrogen use efficiency in high-yielding irrigated cotton. Nutr Cycl Agroecosyst 90:147–156. https://doi.org/10.1007/s10705-010-9418-9
Strand AE, Pritchard SG, McCormack ML, Davis MA, Oren R (2008) Irreconcilable differences: fine-root life spans and soil carbon persistence. Science 319:456–458. https://doi.org/10.1126/science.1151382
Sharma M, Pang J, Wen Z, De Borda A, Kim HS, Liu Y, Lambers H, Ryan MH, Siddique KH (2021) A significant increase in rhizosheath carboxylates and greater specific root length in response to terminal drought is associated with greater relative phosphorus acquisition in chickpea. Plant Soil 460(1):51–68. https://doi.org/10.1007/s11104-020-04776-x
Snider JL, Bange M, Heitholt J (2021a) In book: Crop Physiology Case Histories for Major Crops pp. 714–746. https://doi.org/10.1016/B978-0-12-819194-1.00022-0
Snider J, Harris G, Roberts P, Meeks C, Chastain D, Bange M, Virk G (2021b) Cotton physiological and agronomic response to nitrogen application rate. Field Crops Res 270:108194. https://doi.org/10.1016/j.fcr.2021.108194
Salmon VG, Brice DJ, Bridgham S et al (2021) Nitrogen and phosphorus cycling in an ombrotrophic peatland: a benchmark for assessing change. Plant Soil 466(1):649–674. https://doi.org/10.1007/s11104-021-05065-x
Stevens CJ (2019) Nitrogen in the environment. Science 363(6427):578–580. https://doi.org/10.1126/science.aav8215
Vamerali T, GuariseM GA, Mosca G (2009) Effects of water and nitrogen management on fibrous root distribution and turnover in sugar beet. Eur J Agron 31(2):69–76. https://doi.org/10.1016/j.eja.2009.03.005
Tierney GL, Fahey TJ (2001) Evaluating minirhizotron estimates of fine root longevity and production in the forest floor of a temperate broadleaf forest. Plant Soil 229:167–176. https://doi.org/10.1023/A:1004829423160
Therneau T (2014) A package for survival analysis in S. R package version 2.37-7
Thorup-Kristensen K, Halberg N, Nicolaisen M, Olesen JE, Crews TE, Hinsinger P, John K, Alain P, Dresbøll DB (2020) Digging deeper for agricultural resources, the value of deep rooting. Trends Plant Sci 25(4):406–417. https://doi.org/10.1016/j.tplants.2019.12.007
Tian XM (2016) Cotton Farming Theory and Modern Cultivation Technology in **njiang. Science Press, pp 342–344
Wilcox KR, Shi Z, Gherardi LA et al (2017) Asymmetric responses of primary productivity to precipitation extremes: a synthesis of grassland precipitation manipulation experiments. Global Change Biol 23:4376–4385. https://doi.org/10.1111/gcb.13706
Wu B, Zhang L, Tian J, Zhang G, Zhang W (2022) Nitrogen rate for cotton should be adjusted according to water availability in arid regions. Field Crops Res 285:108606. https://doi.org/10.1016/j.fcr.2022.108606
Wu B, Zhang H, Wang D (2018) Timely supplemental irrigation changed nitrogen use of wheat by regulating root vertical distribution. J Plant Nutr Soil Sci 181:1–13. https://doi.org/10.1002/jpln.201700350
Wang J, Du G, Tian J, Zhang Y, Jiang C, Zhang W (2020) Effect of irrigation methods on root growth, root-shoot ratio and yield components of cotton by regulating the growth redundancy of root and shoot. Agric Water Manag 234:106120. https://doi.org/10.1016/j.agwat.2020.106120
Wang J, Wang Z, Uchida S, Huang W (2009) Relative discrimination of planophile and erectophile wheat types using multi-temporal spectrum measurements. Jpn Agric Res Q 43:157–166. https://doi.org/10.6090/jarq.43.157
Wells CE, Eissenstat DM (2001) Marked differences in survivorship among apple roots of different diameters. Ecology 82:882. https://doi.org/10.2307/2680206
**ao S, Liu L, Zhang Y, Sun H, Zhang K, Bai Z, Dong H, Li C (2020) (2020) Fine root and root hair morphology of cotton under drought stress revealed with RhizoPot. J Agron Crop Sci 206(6):679–693. https://doi.org/10.1111/jac.12429
Zhan A, Schneider H, Lynch JP (2015) Reduced lateral root branching density improves drought tolerance in maize. Plant Physiol 168(4):1603–1615. https://doi.org/10.1104/pp.15.00187
Zhou GY, Zhou XH, Nie YY, Bai SH, Zhou LY, Shao JJ, Cheng WS, Wang JW, Hu FQ, Fu YL (2018) Drought-induced changes in root biomass largely result from altered root morphological traits: evidence from a synthesis of global field trials. Plant Cell Environ 41:2589–2599. https://doi.org/10.1111/pce.13356
Zhu L, Liu L, Sun H, Zhang Y, Liu X, Wang N, Chen J, Zhang K, Bai Z, Wang G, Tian L, Li C (2021) The responses of lateral roots and root hairs to nitrogen stress in cotton based on daily root measurements. J Agron Crop Sci 208(1):89–105. https://doi.org/10.1111/jac.12525
Acknowledgements
This experimental work was supported by the National Key Research and Development Program of China (2018YFD1000907).
Author information
Authors and Affiliations
Contributions
Planning and managing of the project was performed by ZW, TJ, ZG. Field sampling was performed by WB, ZL. Laboratory work was performed by WB and ZL, with the assistance of ZG. Statistical analyses were performed by WB. Writing of the manuscript was performed by WB and ZW.
Corresponding author
Ethics declarations
Ethical approval
Not applicable.
Informed consent
Not applicable.
Conflict of interest
The authors of this manuscript have no competing interests to declare.
Additional information
Responsible Editor: Dorte Bodin Dresbøll.
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Wu, B., Zhang, L., Tian, J. et al. Fine root dynamics, longevity, and canopy characteristics of cotton under varying water and nitrogen levels. Plant Soil 482, 191–209 (2023). https://doi.org/10.1007/s11104-022-05681-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11104-022-05681-1