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Different Characteristics of Hepcidin Expression in IL-6+/+ and IL-6−/− Neurons and Astrocytes Treated with Lipopolysaccharides

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Abstract

A region-specific regulation of inflammation on the expression hepcidin in the brain has been demonstrated, however, it remains unknown whether there is also a cell-specific regulation of inflammation on hepcidin in the brain. Here, we investigated the effects of lipopolysaccharides (LPS) on the expression of hepcidin mRNA and also the expression of IL-6 mRNA, the phosphorylation of STAT3 and the expression of ferroportin 1 (Fpn1) and ferritin light chain (Ft-L) proteins in neurons and astrocytes obtained from wild type (IL-6+/+) and IL-6 knockout (IL-6−/−) mice. We demonstrated that the responses of the expression of hepcidin and IL-6 mRNAs, the phosphorylation of STAT3, and the expression of Fpn1 protein to LPS in IL-6+/+ astrocytes and also the responses of the expression of hepcidin mRNA, the phosphorylation of STAT3 and the expression of Fpn1 protein to IL-6 in IL-6−/− astrocytes were much stronger than those in IL-6+/+ and IL-6−/− neurons. A significant increase in Ft-L was found in LPS-treated IL-6+/+ and IL-6-treated IL-6−/− astrocytes, but not in LPS-treated IL-6+/+ and IL-6-treated IL-6−/− neurons. Our findings provide in vitro evidence for the existence of a cell-specific regulation of LPS on the expression of hepcidin and also Ft-L in the brain.

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Abbreviations

LPS:

Lipopolysaccharides

Fpn1:

Ferroportin 1

Ft-L:

Ferritin light chain

IL-6−/−:

IL-6 knockout

IL-6:

Interleukin-6

STAT3:

Signal transducer and activator of transcription 3

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Acknowledgements

The studies in our laboratories were supported by National Natural Science Foundation of China (31330035, 31571195), and the National Basic Research Program of China (973) (2014CB541604).

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Authors and Affiliations

  1. Laboratory of Neuropharmacology, Fudan University School of Pharmacy, 826 Zhang Heng Road, Shanghai, 201203, People’s Republic of China

    Juan Ma, Fa-Li Zhang, Yuan Shen & Zhong-Ming Qian

  2. Laboratory of Neuropharmacology, Institute of Translational and Precision Medicine, Nantong University, Nantong, 226001, People’s Republic of China

    Zhong-Ming Qian

  3. Department of Clinical Pharmacology, **angya Hospital, Central South University, Changsha, 410008, People’s Republic of China

    Gan Zhou

  4. Research Center for Medicine and Biology, Zunyi Medical University, Zunyi, 563000, Guizhou, People’s Republic of China

    Yu-**n Bao

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  1. Juan Ma
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Contributions

GZ, YXB and ZMQ conceived, organized and supervised the study; JM, FLZ and YS performed the experiments; YXB and GZ contributed to the analysis of data; ZMQ prepared and wrote the manuscript. All authors read and approved the final edition of the manuscript.

Corresponding authors

Correspondence to Gan Zhou, Yu-**n Bao or Zhong-Ming Qian.

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The authors who have taken part in this study declared that they do not have anything to disclose regarding funding or conflict of interest with respect to this manuscript.

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Ma, J., Zhang, FL., Zhou, G. et al. Different Characteristics of Hepcidin Expression in IL-6+/+ and IL-6−/− Neurons and Astrocytes Treated with Lipopolysaccharides. Neurochem Res 43, 1624–1630 (2018). https://doi.org/10.1007/s11064-018-2577-9

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  • DOI: https://doi.org/10.1007/s11064-018-2577-9

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