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Ciliary Neurotrophic Factor-treated Astrocyte Conditioned Medium Regulates the L-type Calcium Channel Activity in Rat Cortical Neurons

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Abstract

Astrocytes are activated by ciliary neurotrophic factor (CNTF) in vivo and in vitro, however, the consequences on the L-type calcium channel (LCC) of neurons are still poorly understood. Therefore, in the present study, whole-cell patch clamp, western-blot and RT-PCR assay were performed to evaluate the effects of CNTF-treated astrocyte conditioned medium (CNTF-ACM) on LCC current (ICa-L) and the expression of Cav1.2 and Cav1.3 in Sprague–Dawley rat cortical neurons. The results revealed that CNTF-ACM enhanced the amplitude of Ica-L and the expression of Cav1.3 significantly, but had no effects on Cav1.2 expression. We also found an increase in the concentration of fibroblast growth factor-2 (FGF-2) in CNTF-ACM by ELISA assay. Taken together, these findings indicate that CNTF induces the release of factors, including FGF-2, from astrocytes, thereby potentiating the activity of LCC in cortical neurons.

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Acknowledgments

This work was supported by National Key Natural Science Foundation of China (grant numbers: 30230140) and Doctor Point Foundation of Ministry of Education of China (grant number 20040487060).

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Authors and Affiliations

  1. Department of Anatomy, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Rd, Wuhan, Hubei, 430030, P.R. China

    **ao**g Wang, Honghua Zheng, Changgeng Zhu & Zhengli Li

  2. Department of Anatomy, Xuzhou Medical College, Xuzhou, 221004, P.R. China

    **ao**g Wang

  3. Department of Physiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, P.R. China

    Chang** Liu

  4. Department of Neurology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, 430030, P.R. China

    Wei Wang

Authors
  1. **ao**g Wang
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  2. Honghua Zheng
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  3. Chang** Liu
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  4. Changgeng Zhu
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  5. Wei Wang
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  6. Zhengli Li
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Correspondence to Zhengli Li.

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**ao**g Wang and Honghua Zheng contributed equally.

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Wang, X., Zheng, H., Liu, C. et al. Ciliary Neurotrophic Factor-treated Astrocyte Conditioned Medium Regulates the L-type Calcium Channel Activity in Rat Cortical Neurons. Neurochem Res 33, 826–832 (2008). https://doi.org/10.1007/s11064-007-9514-7

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  • DOI: https://doi.org/10.1007/s11064-007-9514-7

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