Confocal microscopy was used to analyze double immunolabeling and provided evidence of the locations of a large number of tyrosine hydroxylase-immunopositive processes around the bodies of orexinergic neurons located in the perifornical area of the hypothalamus in rats. The bodies of orexinergic neurons were found to bear dopamine D1 receptors. A high level of colocalization of dopamine D1 and D2 receptors was found in the perifornical area, which is evidence for the formation of heterodimeric D1/D2 complexes. After i.p. administration of the selective D1 receptor antagonist SCH 39166, neurons in the perifornical area showed increases in the optical density of c-Fos protein in both orexinergic neurons and their adjacent GABA neurons. These data provide evidence that dopamine is able to influence orexinergic neurons both via a direct route involving D1- and D2-dependent signal pathways and via influences on GABA neurons.
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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 104, No. 6, pp. 692–700, June, 2018.
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Morina, I.Y., Mikhrina, A.L. & Romanova, I.V. An Immunohistochemical Study of the Pathways of the Influence of Dopamine on Orexinergic Neurons in the Perifornical Area of the Hypothalamus. Neurosci Behav Physi 49, 1100–1105 (2019). https://doi.org/10.1007/s11055-019-00846-5
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DOI: https://doi.org/10.1007/s11055-019-00846-5