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Facile fabrication of a stable and recyclable lipase@amine-functionalized ZIF-8 nanoparticles for esters hydrolysis and transesterification

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Abstract

Zeolitic imidazolate frameworks (ZIF) represent one of the metal organic frameworks (MOF) with high potential for enzyme immobilization due to their exceptional chemical and thermal stability, negligible cytotoxicity, and easy synthesis under mild biocompatible conditions. Amine-functionalized ZIF-8 (An-ZIF-8) are capable of forming multipoint attachment via hydrogen bonding with lipase which will immobilize and further enhance stabilization of lipase. In addition, increased hydrophilicity of An-ZIF-8 will increase partitioning of An-ZIF-8 immobilized lipase at the aqueous/organic interface which enable lipase to expose its active site and retain its catalytic activity at its highest. Present study reports the use of ZIF-8 and An-ZIF-8 nanoparticles as carrier for Burkholderia cepacia lipase (BCL), compares the ester hydrolysis and transesterification activities of immobilized lipase with those of free lipase, and evaluates the reusability and recovery rate of the immobilized lipase. An-ZIF-8 nanoparticles (average 130.42 ± 0.55 nm) were facilely synthesized via mixing ZIF-8 nanoparticles with ammonia hydroxide solution. Despite having similar characteristics of high crystallinity and forming cuboid-like particles, An-ZIF-8 demonstrated significantly (P < 0.05) lower Brunauer-Emmett-Teller (BET) surface area and higher thermal stability than ZIF-8. BCL were successfully immobilized on ZIF-8 (BCL@ZIF-8) and An-ZIF-8 (BCL@An-ZIF-8) nanoparticles with an average lipase loading rate of ~ 8 mg/g MOF. The immobilized BCL demonstrated no significant differences in terms of esters hydrolysis and transesterification activities with those of free BCL. BCL@An-ZIF-8 demonstrated superior catalytic stability in comparison to BCL@ZIF-8 with retainment of more than 80% of its initial hydrolysis and transesterification activity for at least 10 repeated runs. In addition, more than 80% of the BCL@An-ZIF-8 can be easily recovered during each cycle of the reusability test through simple centrifugation.

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Acknowledgements

This work was sponsored by K.C.Wong Magna Fund in Ningbo University. Ling-Zhi Cheong acknowledged funding support from Ningbo University (421401560).

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  1. Ling-Zhi Cheong and Yayu Wei contributed equally to this work.

Authors and Affiliations

  1. Department of Food Safety and Quality, School of Marine Science, Ningbo University, Ningbo, 315211, China

    Ling-Zhi Cheong, Hongbin Wang, Zhiying Wang & **urong Su

  2. Department of Materials Science and Engineering, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, China

    Yayu Wei

  3. Chinese Academy of Sciences, Ningbo Institute of Materials Technology & Engineering, 1219 Zhongguan Road, Ningbo, 315201, China

    Yayu Wei & Cai Shen

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  1. Ling-Zhi Cheong
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  2. Yayu Wei
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  4. Zhiying Wang
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  5. **urong Su
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Correspondence to Ling-Zhi Cheong or Cai Shen.

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Cheong, LZ., Wei, Y., Wang, H. et al. Facile fabrication of a stable and recyclable lipase@amine-functionalized ZIF-8 nanoparticles for esters hydrolysis and transesterification. J Nanopart Res 19, 280 (2017). https://doi.org/10.1007/s11051-017-3979-3

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