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Systematic analyzing a five- miRNA panel and its diagnostic value of plasma expression in colorectal cancer

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Abstract

Background

Aberrant expression of miRNAs have been implicated in cancers, but the role of miRNAs in colorectal cancer (CRC) remains need to be elucidated. This study aimed to identify miRNAs that related to colorectal cancer (CRC) pathogenesis and determine the diagnostic value.

Methods

Three GEO datasets (GSE128449, GSE35602 and GSE49246) with 131 samples were used to screen miRNAs that differential expression between tumor and control tissues. The expression of the identified miRNAs was validated in 50 clinical tissue samples and the GSE35834 dataset. The clinical significance of these miRNAs was analyzed in the TCGA dataset and clinical tissue samples. The expression of miRNAs in tissues and plasma samples were tested by RT-PCR assay in clinical samples, and their diagnostic value was determined.

Results

The analysis of three GEO datasets revealed that miR-595 and miR-1237 were upregulated, while miR-126, miR-139, and miR-143 were downregulated in CRC tissues compared to control tissues. The differential expression of the five miRNAs in CRC tissues was confirmed using clinical tissue samples and GEO databases. There was no significant correlation between the TNM stage and tumor stage of CRC and any of the five miRNAs. Plasma expression of the miRNAs differed significantly between CRC and non-cancer patients, and each miRNA had moderate diagnostic value for CRC. Combining the five miRNAs provided better diagnostic potential for CRC than a single miRNA.

Conclusions

This study demonstrated that five miRNAs were related to the pathogenesis of CRC, but independent of the stage of CRC; Plasma expression of these miRNAs have moderate diagnostic value, and combination of these miRNAs showed better diagnostic ability in CRC.

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Data availability

The data used to support the findings of this study are available from GEO databases (www.ncbi.nlm.nih.gov/geo/), with the access number as GSE128449, GSE35602, GSE49246, GSE32323, GSE35834, and GSE59856 respectively. The TCGA-COADREAD datasets was downloaded from Xena database (https://xenabrowser.net/datapages/).

Abbreviations

CRC:

Colorectal cancer

DEMs:

Differential expression miRNAs

ROC:

Receiver Operating Characteristic

AUC:

Area Under ROC Curve

RT-PCR:

Reverse transcription-polymerase chain reaction

CEA:

Carcinoembryonic antigen

CA125:

Carbohydrate antigen 125

CA19-9:

Carbohydrate antigen 19–9

CA153:

Carbohydrate antigen 153

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Acknowledgements

Not applicable

Funding

This study was partially supported by National Natural Science Foundation of China (No. 82160446 and 82260580); Guangxi Medical and health key discipline construction project; the Training Program for 1000 Young and Middle-aged Key Teachers in Guangxi; Scientific Research Project of Guangxi Health Commission (No. Z20190717).

Author information

Author notes

  1. Si-Qi Li and Li-Ye **e contributed equally.

Authors and Affiliations

  1. Department of Research, Guangxi Medical University Cancer Hospital, No. 71 Hedi Road, Nanning, 530021, China

    Si-Qi Li, Li-Ye **e, Zheng-Min Cai, Bang-Li Hu & Shu-Fang Ning

  2. Department of Gastrointestinal Surgery, Third Affiliated Hospital of Guangxi Medical University, Nanning, 530031, China

    Hao-Tang Wei

  3. Department of Chemotherapy, Guangxi Medical University Cancer Hospital, Nanning, 530031, China

    Ming-Zhi **e

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Contributions

Study concept and design: NSF and HBL; Collection and assembly of data: LSQ, XLY, CZM and XMZ; Performed the experiment: LSQ, XLY and CZM; Data analysis and interpretation: WHT, HBL, NSF and LSQ; Manuscript writing and review: All authors. All authors have read and approved the manuscript in its current state.

Corresponding authors

Correspondence to Bang-Li Hu or Shu-Fang Ning.

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The authors declare that they have no conflict of interest.

Ethics approval and consent to participate

All the procedures were carried out in accordance with institutional guidelines. The study is approved by the Ethics Committee of Guangxi Medical University Cancer Hospital (2021-KY-NSFC-042), written informed consent was obtained from each patient. All the procedures were carried out in accordance with Declaration of Helsinki.

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Li, SQ., **e, LY., Cai, ZM. et al. Systematic analyzing a five- miRNA panel and its diagnostic value of plasma expression in colorectal cancer. Mol Biol Rep 50, 7253–7261 (2023). https://doi.org/10.1007/s11033-023-08642-8

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  • DOI: https://doi.org/10.1007/s11033-023-08642-8

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