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MicroRNAs expression in peripheral blood mononuclear cells of patients with multiple sclerosis propose

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Abstract

Background

MicroRNAs (miRs) are involved in the autoimmune and neurological diseases, including multiple sclerosis (MS), through modulating post-transcriptional gene regulation. Accumulating evidence indicates that miR-10, miR-24a, miR-124, and miR-21 play an imperative role in MS pathogenesis. Therefore, the current research aimed to analyze the expression of the selected miRNAs for MS in Iranian population.

Methods and Results

Blood sample of 75 relapsing-remitting MS (RRMS) patients and 75 healthy individuals suffering no neurodegenerative illness was collected. Subsequently, the isolation of peripheral blood mononuclear cells (PBMCs) was performed by employing Ficoll-Hypaque density gradient method. Afterward, total RNA was extracted and subjected to qRT-PCR analysis. The obtained results evidenced that the relative expression of miR-10 (P = 0.0002), miR-21 (P = 0.0014), and miR-124 (P = 0.0091) significantly decreased in RRMS patients compared to healthy participants. On the contrary, no notable change was observed between the studies groups regarding miR-24a expression levels (P = 0.107). ROC curve analysis estimated an area under the curve (AUC) value equal to 0.75 with P = 0.0006 for miR-10, while it was decreased for miR-21 (AUC = 0.67 and P = 0.0054) and miR-124 (AUC = 0.66 and P = 0.012).

Conclusion

The change in miR-10, miR-124, and miR-21 expression patterns was implied to participate in MS development. Further large scale observational studies are recommended.

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Data availability

The datasets generated and/or analyzed during the current study are not publicly available due for they are personal data but are available from the corresponding author on reasonable request.

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Acknowledgements

The authors are grateful of patients, healthy individuals, and their family who contributed to accomplishment of this research.

Funding

None.

Author information

Authors and Affiliations

  1. Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Mahsa Abolghasemi & Elham Poursaei

  2. Neurosciences Research Center (NSRC), Tabriz University of Medical Sciences, Tabriz, Iran

    Sepide Ali Ashrafi, Saeed Sadigh Etehad & Sheida Shaafi

  3. Department of Basic Oncology, Health Institute of Ege University, Izmir, Turkey

    Milad Asadi

  4. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Dariush Shanehbandi

  5. Research Center for Integrative Medicine in Aging, Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran

    Seyed Aria Nejadghaderi

Authors
  1. Mahsa Abolghasemi
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  2. Sepide Ali Ashrafi
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  3. Milad Asadi
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  4. Dariush Shanehbandi
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  5. Saeed Sadigh Etehad
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  7. Seyed Aria Nejadghaderi
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  8. Sheida Shaafi
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Contributions

M.A.G. and S.S.: designed the study. M.A.G., S.A.A., M.A., D.S., and S.S.: patient data acquisition, statistical analysis, and interpretation of data. D.S., S.S.E., E.P., and S.A.N.: preparation and critically revision of the manuscript. All the authors have read and approved the final version of the manuscript.

Corresponding author

Correspondence to Sheida Shaafi.

Ethics declarations

Ethics approval and consent to participate

Written informed consent was obtained from all of the participants at the beginning of the study. The study protocol was approved by the ethics committee of Tabriz University of Medical Sciences (Ethics number: IR.TBZMED.REC.1400.433). All methods were performed in accordance with the national guidelines and regulations and the ethical standards of the Declaration of Helsinki 1964.

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The authors have declared that no competing financial interests or personal relationships exists in this paper.

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Abolghasemi, M., Ali Ashrafi, S., Asadi, M. et al. MicroRNAs expression in peripheral blood mononuclear cells of patients with multiple sclerosis propose. Mol Biol Rep 50, 167–172 (2023). https://doi.org/10.1007/s11033-022-07905-0

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